Display options
Cite
Jühling F, Saviano A, Ponsolles C, et al. Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinoma. Life Sci Alliance. 2021;4(9)doi: 10.26508/lsa.202101036.
Jühling, F., Saviano, A., Ponsolles, C., Heydmann, L., Crouchet, E., Durand, S. C., El Saghire, H., Felli, E., Lindner, V., Pessaux, P., Pochet, N., Schuster, C., Verrier, E. R., & Baumert, T. F. (2021). Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinoma. Life science alliance, 4(9), . https://doi.org/10.26508/lsa.202101036
Jühling, Frank, et al. "Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinoma." Life science alliance vol. 4,9 (2021). doi: https://doi.org/10.26508/lsa.202101036
Jühling F, Saviano A, Ponsolles C, Heydmann L, Crouchet E, Durand SC, El Saghire H, Felli E, Lindner V, Pessaux P, Pochet N, Schuster C, Verrier ER, Baumert TF. Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinoma. Life Sci Alliance. 2021 Jul 21;4(9). doi: 10.26508/lsa.202101036. Print 2021 Sep. PMID: 34290079; PMCID: PMC8321681.
Copy Download .nbib Format: NLM
Share it on

Life Sci Alliance. 2021 Jul 21;4(9). doi: 10.26508/lsa.202101036. Print 2021 Sep.

Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinoma.

Life science alliance

Frank Jühling, Antonio Saviano, Clara Ponsolles, Laura Heydmann, Emilie Crouchet, Sarah C Durand, Houssein El Saghire, Emanuele Felli, Véronique Lindner, Patrick Pessaux, Nathalie Pochet, Catherine Schuster, Eloi R Verrier, Thomas F Baumert

Affiliations

  1. Université de Strasbourg, Inserm, Institut de Recherche sur Les Maladies Virales et Hépatiques UMR_S1110, Strasbourg, France.
  2. Institut Hospitalo-Universitaire, Pôle Hépato-digestif, Nouvel Hôpital Civil, Strasbourg, France.
  3. Hôpitaux Universitaires de Strasbourg, Département de Pathologie, Strasbourg, France.
  4. Ann Romney Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  5. Cell Circuits Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  6. Université de Strasbourg, Inserm, Institut de Recherche sur Les Maladies Virales et Hépatiques UMR_S1110, Strasbourg, France [email protected].
  7. Université de Strasbourg, Inserm, Institut de Recherche sur Les Maladies Virales et Hépatiques UMR_S1110, Strasbourg, France [email protected].
  8. Institut Universitaire de France (IUF), Paris, France.

PMID: 34290079 PMCID: PMC8321681 DOI: 10.26508/lsa.202101036

Abstract

Chronic hepatitis B virus (HBV) infection is a major cause of hepatocellular carcinoma (HCC) world-wide. The molecular mechanisms of viral hepatocarcinogenesis are still partially understood. Here, we applied two complementary single-cell RNA-sequencing protocols to investigate HBV-HCC host cell interactions at the single cell level of patient-derived HCC. Computational analyses revealed a marked HCC heterogeneity with a robust and significant correlation between HBV reads and cancer cell differentiation. Viral reads significantly correlated with the expression of HBV-dependency factors such as

© 2021 Jühling et al.

References

  1. Bioinformatics. 2015 Jan 15;31(2):166-9 - PubMed
  2. J Clin Invest. 2017 Mar 1;127(3):830-842 - PubMed
  3. Nature. 2014 Jun 19;510(7505):363-9 - PubMed
  4. Cancer Res. 2008 Aug 15;68(16):6779-88 - PubMed
  5. J Hepatol. 2016 Apr;64(1 Suppl):S84-S101 - PubMed
  6. Cell Syst. 2015 Dec 23;1(6):417-425 - PubMed
  7. Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4579-84 - PubMed
  8. Nat Commun. 2018 Oct 22;9(1):4383 - PubMed
  9. J Virol. 2015 Dec 04;90(4):1729-40 - PubMed
  10. Curr Opin Virol. 2014 Feb;4:71-7 - PubMed
  11. J Mol Med (Berl). 2016 Jun;94(6):613-27 - PubMed
  12. Nat Protoc. 2014 Jan;9(1):171-81 - PubMed
  13. Nat Methods. 2018 May;15(5):379-386 - PubMed
  14. Science. 2015 Jan 23;347(6220):1260419 - PubMed
  15. Gastroenterology. 2009 Mar;136(3):1012-24 - PubMed
  16. Hepatology. 2007 Jan;45(1):42-52 - PubMed
  17. Viruses. 2020 Jan 30;12(2): - PubMed
  18. Hepatology. 1996 Aug;24(2):289-93 - PubMed
  19. Nature. 1979 Dec 6;282(5739):615-6 - PubMed
  20. Gastroenterology. 2012 May;142(6):1264-1273.e1 - PubMed
  21. Nat Methods. 2014 Jan;11(1):22-4 - PubMed
  22. Cancer. 1954 May;7(3):462-503 - PubMed
  23. Nat Methods. 2013 Nov;10(11):1096-8 - PubMed
  24. Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15655-60 - PubMed
  25. World J Gastroenterol. 2015 Nov 14;21(42):12171-8 - PubMed
  26. Cell Signal. 2015 Jul;27(7):1544-55 - PubMed
  27. Curr Protoc Mol Biol. 2014 Jul 01;107:4.22.1-17 - PubMed
  28. Biochem Biophys Res Commun. 2018 Sep 18;503(4):2885-2891 - PubMed
  29. Int J Mol Sci. 2016 Aug 22;17(8): - PubMed
  30. J Hepatol. 2006 Oct;45(4):529-38 - PubMed
  31. Clin Cancer Res. 2014 Aug 1;20(15):4129-40 - PubMed
  32. Cancer Res. 2009 Sep 15;69(18):7385-92 - PubMed
  33. Sci Rep. 2015 Mar 26;5:9536 - PubMed
  34. Hepatology. 2010 Apr;51(4):1144-57 - PubMed
  35. Hepatology. 2018 Jan;67(1):86-96 - PubMed
  36. Antiviral Res. 2019 Dec;172:104618 - PubMed
  37. Cell Microbiol. 2008 Jul;10(7):1478-90 - PubMed
  38. Science. 2017 Aug 18;357(6352): - PubMed
  39. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50 - PubMed
  40. Clin Liver Dis. 2007 Nov;11(4):797-816, viii - PubMed
  41. Clin Med Insights Oncol. 2014 May 19;8:71-6 - PubMed
  42. Sci Rep. 2015 Jul 02;5:10566 - PubMed
  43. J Hepatol. 2017 Jan;66(1):28-38 - PubMed
  44. Mol Cancer. 2015 Sep 17;14:170 - PubMed
  45. BMC Bioinformatics. 2013 Jan 16;14:7 - PubMed
  46. Nat Commun. 2020 Jan 15;11(1):291 - PubMed
  47. Eur J Cancer. 2011 Feb;47(3):333-8 - PubMed
  48. Cell Syst. 2016 Oct 26;3(4):385-394.e3 - PubMed
  49. Nat Genet. 2013 Oct;45(10):1113-20 - PubMed
  50. Physiol Genomics. 2001 Dec 21;7(2):97-104 - PubMed
  51. CA Cancer J Clin. 2018 Nov;68(6):394-424 - PubMed
  52. Nature. 2019 Aug;572(7768):199-204 - PubMed
  53. Hepatology. 2016 Jan;63(1):35-48 - PubMed
  54. Cell Stem Cell. 2016 Aug 4;19(2):266-277 - PubMed
  55. J Immunol. 2016 Jul 15;197(2):630-43 - PubMed
  56. Hepat Mon. 2013 May 27;13(5):e8792 - PubMed
  57. Nature. 2019 Feb;566(7744):388-392 - PubMed
  58. Nat Methods. 2020 Jan;17(1):45-49 - PubMed
  59. J Med Virol. 2014 Apr;86(4):687-94 - PubMed
  60. Cancer Res. 2011 Apr 15;71(8):2949-58 - PubMed

Publication Types

Grant support