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Yang H, Cao Z, Wang Y, et al. Treadmill exercise influences the microRNA profiles in the bone tissues of mice. Exp Ther Med. 2021;22(3):1035doi: 10.3892/etm.2021.10467.
Yang, H., Cao, Z., Wang, Y., Wang, J., Gao, J., Han, B., Yu, F., Qin, Y., & Guo, Y. (2021). Treadmill exercise influences the microRNA profiles in the bone tissues of mice. Experimental and therapeutic medicine, 22(3), 1035. https://doi.org/10.3892/etm.2021.10467
Yang, Huan, et al. "Treadmill exercise influences the microRNA profiles in the bone tissues of mice." Experimental and therapeutic medicine vol. 22,3 (2021): 1035. doi: https://doi.org/10.3892/etm.2021.10467
Yang H, Cao Z, Wang Y, Wang J, Gao J, Han B, Yu F, Qin Y, Guo Y. Treadmill exercise influences the microRNA profiles in the bone tissues of mice. Exp Ther Med. 2021 Sep;22(3):1035. doi: 10.3892/etm.2021.10467. Epub 2021 Jul 19. PMID: 34373721; PMCID: PMC8343800.
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Exp Ther Med. 2021 Sep;22(3):1035. doi: 10.3892/etm.2021.10467. Epub 2021 Jul 19.

Treadmill exercise influences the microRNA profiles in the bone tissues of mice.

Experimental and therapeutic medicine

Huan Yang, Zhen Cao, Yang Wang, Jiahui Wang, Jintao Gao, Biao Han, Fangmei Yu, Yixiong Qin, Yong Guo

Affiliations

  1. Department of Biomedical Engineering, College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
  2. Department of Biomedical Engineering, Bioengineering College of Chongqing University, Chongqing 400044, P.R. China.

PMID: 34373721 PMCID: PMC8343800 DOI: 10.3892/etm.2021.10467

Abstract

As an important regulator involved in cell activity, microRNAs (miRNAs) are important in the process of exercise influencing bone metabolism. The present study aimed to detect and select differentially expressed miRNAs in the bone tissues of mice trained on a treadmill, predict the target genes of these differentially expressed miRNAs and lay a foundation for exploring the effect of treadmill training on bone metabolism through miRNAs. In this experiment, after the mice were trained on a treadmill for 8 weeks, the mechanical properties of mouse femur bone were assessed, and the alkaline phosphatase (ALP) activity and osteocalcin (OCN) protein levels of the bone were assayed. miRNA microarray and reverse transcription-quantitative (RT-q)PCR were performed to select and validate differentially expressed miRNAs in the bone, and the target genes of these miRNAs were predicted with bioinformatics methods. In addition, the differentially expressed miRNAs in the bone tissues were compared with those in mechanically strained osteocytes

Copyright: © Yang et al.

Keywords: bioinformatics; bone; microRNA; osteocyte; treadmill exercise

Conflict of interest statement

The authors declare that they have no competing interests.

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