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Zelco A, Börjesson V, de Kanter JK, et al. Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain. Genes Dev. 2021;35(15):1190-1207doi: 10.1101/gad.348190.120.
Zelco, A., Börjesson, V., de Kanter, J. K., Lebrero-Fernandez, C., Lauschke, V. M., Rocha-Ferreira, E., Nilsson, G., Nair, S., Svedin, P., Bemark, M., Hagberg, H., Mallard, C., Holstege, F. C. P., & Wang, X. (2021). Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain. Genes & development, 35(15), 1190-1207. https://doi.org/10.1101/gad.348190.120
Zelco, Aura, et al. "Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain." Genes & development vol. 35,15 (2021): 1190-1207. doi: https://doi.org/10.1101/gad.348190.120
Zelco A, Börjesson V, de Kanter JK, Lebrero-Fernandez C, Lauschke VM, Rocha-Ferreira E, Nilsson G, Nair S, Svedin P, Bemark M, Hagberg H, Mallard C, Holstege FCP, Wang X. Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain. Genes Dev. 2021 Aug 01;35(15):1190-1207. doi: 10.1101/gad.348190.120. Epub 2021 Jul 22. PMID: 34301765; PMCID: PMC8336895.
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Genes Dev. 2021 Aug 01;35(15):1190-1207. doi: 10.1101/gad.348190.120. Epub 2021 Jul 22.

Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain.

Genes & development

Aura Zelco, Vanja Börjesson, Jurrian K de Kanter, Cristina Lebrero-Fernandez, Volker M Lauschke, Eridan Rocha-Ferreira, Gisela Nilsson, Syam Nair, Pernilla Svedin, Mats Bemark, Henrik Hagberg, Carina Mallard, Frank C P Holstege, Xiaoyang Wang

Affiliations

  1. Centre of Perinatal Medicine and Health, Institute of Neuroscience and Physiology, Department of Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 40530, Sweden.
  2. Bioinformatics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg 413 90, Sweden.
  3. Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands.
  4. Department of Microbiology and Immunology, Sahlgrenska Academy, University of Gothenburg, Gothenburg 40530, Sweden.
  5. Department of Physiology and Pharmacology, Karolinska Institute, Stockholm 17177, Sweden.
  6. Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart 70 376, Germany.
  7. Centre of Perinatal Medicine and Health, Institute of Clinical Sciences, Department of Obstetrics and Gynecology, Sahlgrenska Academy, Gothenburg University, Gothenburg 40530, Sweden.
  8. Henan Key Laboratory of Child Brain Injury, Institute of Neuroscience, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

PMID: 34301765 PMCID: PMC8336895 DOI: 10.1101/gad.348190.120

Abstract

The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. We identified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.

© 2021 Zelco et al.; Published by Cold Spring Harbor Laboratory Press.

Keywords: meningeal leukocytes; neonatal mouse; preterm brain injury; single-cell RNA sequencing

References

  1. J Exp Med. 2017 Feb;214(2):285-296 - PubMed
  2. Brain Res Dev Brain Res. 1995 Feb 16;84(2):245-52 - PubMed
  3. J Cereb Blood Flow Metab. 2007 Nov;27(11):1798-805 - PubMed
  4. J Biol Chem. 2012 Aug 17;287(34):28738-44 - PubMed
  5. J Biol Chem. 2016 Jan 29;291(5):2389-96 - PubMed
  6. Eur J Immunol. 2015 Jan;45(1):214-24 - PubMed
  7. Ann Neurol. 2016 Jul;80(1):24-34 - PubMed
  8. Neurosci Lett. 2009 Oct 16;464(1):29-33 - PubMed
  9. J Leukoc Biol. 2011 Aug;90(2):221-33 - PubMed
  10. Front Pharmacol. 2017 Oct 27;8:766 - PubMed
  11. Science. 2021 Jun 3;: - PubMed
  12. Neuron. 2019 Jan 16;101(2):207-223.e10 - PubMed
  13. J Biol Chem. 2004 Apr 23;279(17):17625-33 - PubMed
  14. Pediatr Neurol. 2019 Jun;95:42-66 - PubMed
  15. J Neuroimmunol. 1990 Nov;30(1):81-93 - PubMed
  16. J Neurosci. 2012 Aug 15;32(33):11285-98 - PubMed
  17. Blood. 2006 Dec 1;108(12):3682-90 - PubMed
  18. PLoS One. 2017 Jan 12;12(1):e0170261 - PubMed
  19. Cell Metab. 2019 Sep 3;30(3):493-507.e6 - PubMed
  20. Front Immunol. 2019 Sep 20;10:2199 - PubMed
  21. Acta Neuropathol. 2017 Sep;134(3):331-349 - PubMed
  22. Immunity. 2019 Jan 15;50(1):253-271.e6 - PubMed
  23. J Neurosci. 2001 Feb 15;21(4):1302-12 - PubMed
  24. N Engl J Med. 2020 Jan 16;382(3):233-243 - PubMed
  25. Circulation. 2006 May 2;113(17):2105-12 - PubMed
  26. Nat Immunol. 2020 Nov;21(11):1421-1429 - PubMed
  27. Annu Rev Neurosci. 2012;35:369-89 - PubMed
  28. Neuron. 2012 Feb 23;73(4):698-712 - PubMed
  29. Neuroscience. 2013 May 15;238:242-51 - PubMed
  30. Front Pediatr. 2016 Oct 20;4:114 - PubMed
  31. Pediatr Res. 1988 Aug;24(2):213-6 - PubMed
  32. Cell. 2020 Apr 30;181(3):557-573.e18 - PubMed
  33. J Neuroimmunol. 2015 Jan 15;278:280-8 - PubMed
  34. Cell. 2007 Dec 14;131(6):1164-78 - PubMed
  35. Stroke. 2015 Oct;46(10):3006-11 - PubMed
  36. Dev Cell. 2020 Jul 6;54(1):43-59.e4 - PubMed
  37. Cell Mol Immunol. 2011 Jul;8(4):305-14 - PubMed
  38. Sci Immunol. 2019 Oct 11;4(40): - PubMed
  39. Immunity. 2018 Feb 20;48(2):380-395.e6 - PubMed
  40. Nat Rev Neurol. 2019 Aug;15(8):447-458 - PubMed
  41. Front Neurol. 2018 Mar 19;9:159 - PubMed
  42. Eur J Clin Invest. 2018 Nov;48 Suppl 2:e12949 - PubMed
  43. Nucleic Acids Res. 2019 Sep 19;47(16):e95 - PubMed
  44. Front Immunol. 2018 Aug 06;9:1696 - PubMed
  45. Am J Pathol. 2018 Mar;188(3):757-767 - PubMed
  46. Nat Neurosci. 2018 Apr;21(4):506-516 - PubMed
  47. Cell. 2009 Oct 30;139(3):597-609 - PubMed
  48. J Neuroimmunol. 2003 Mar;136(1-2):84-93 - PubMed
  49. Oral Oncol. 2015 Mar;51(3):221-8 - PubMed
  50. Nat Cell Biol. 2018 Jul;20(7):836-846 - PubMed
  51. Nat Immunol. 2006 Jul;7(7):732-9 - PubMed
  52. Nat Neurosci. 2019 Jun;22(6):1021-1035 - PubMed
  53. Front Cell Neurosci. 2020 Aug 07;14:249 - PubMed
  54. J Neuroinflammation. 2017 Dec 20;14(1):255 - PubMed
  55. Life Sci Alliance. 2020 Dec 4;4(1): - PubMed
  56. Nat Med. 2009 Aug;15(8):946-50 - PubMed
  57. Neuron. 2012 May 24;74(4):691-705 - PubMed

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