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Fisher F, Zhang Y, Vincent PFY, et al. Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells. Front Cell Neurosci. 2021;15:697560doi: 10.3389/fncel.2021.697560.
Fisher, F., Zhang, Y., Vincent, P. F. Y., Gajewiak, J., Gordon, T. J., Glowatzki, E., Fuchs, P. A., & McIntosh, J. M. (2021). Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells. Frontiers in cellular neuroscience, 15697560. https://doi.org/10.3389/fncel.2021.697560
Fisher, Fernando, et al. "Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells." Frontiers in cellular neuroscience vol. 15 (2021): 697560. doi: https://doi.org/10.3389/fncel.2021.697560
Fisher F, Zhang Y, Vincent PFY, Gajewiak J, Gordon TJ, Glowatzki E, Fuchs PA, McIntosh JM. Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells. Front Cell Neurosci. 2021 Jul 23;15:697560. doi: 10.3389/fncel.2021.697560. eCollection 2021. PMID: 34385908; PMCID: PMC8354143.
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Front Cell Neurosci. 2021 Jul 23;15:697560. doi: 10.3389/fncel.2021.697560. eCollection 2021.

Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.

Frontiers in cellular neuroscience

Fernando Fisher, Yuanyuan Zhang, Philippe F Y Vincent, Joanna Gajewiak, Thomas J Gordon, Elisabeth Glowatzki, Paul Albert Fuchs, J Michael McIntosh

Affiliations

  1. Department of Biology, University of Utah, Salt Lake City, UT, United States.
  2. The Center for Hearing and Balance, Otolaryngology-Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, United States.
  3. George E. Wahlen Department of Veterans Affairs Medical Center, Salt Lake City, UT, United States.
  4. Department of Psychiatry, University of Utah School Medicine, Salt Lake City, UT, United States.

PMID: 34385908 PMCID: PMC8354143 DOI: 10.3389/fncel.2021.697560

Abstract

Efferent cholinergic neurons inhibit sensory hair cells of the vertebrate inner ear through the combined action of calcium-permeable α9α10-containing nicotinic acetylcholine receptors (nAChRs) and associated calcium-dependent potassium channels. The venom of cone snails is a rich repository of bioactive peptides, many with channel blocking activities. The conopeptide analog, RgIA-5474, is a specific and potent antagonist of α9α10-containing nAChRs. We added an alkyl functional group to the N-terminus of the RgIA-5474, to enable click chemistry addition of the fluorescent cyanine dye, Cy3. The resulting peptide, Cy3-RgIA-5727, potently blocked mouse α9α10 nAChRs expressed in

Copyright © 2021 Fisher, Zhang, Vincent, Gajewiak, Gordon, Glowatzki, Fuchs and McIntosh.

Keywords: Xenopus; cochlea; conotoxin; mouse; nAChR

Conflict of interest statement

Certain conopeptides have been patented by the University of Utah; JG and JM are inventors on these patents. The remaining authors declare that the research was conducted in the absence of any commerc

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