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Xiang M, Wang L, Yan Q, et al. Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making. Biotechnol Lett. 2021;43(9):1921-1932doi: 10.1007/s10529-021-03167-1.
Xiang, M., Wang, L., Yan, Q., Jiang, Z., & Yang, S. (2021). Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making. Biotechnology letters, 43(9), 1921-1932. https://doi.org/10.1007/s10529-021-03167-1
Xiang, Man, et al. "Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making." Biotechnology letters vol. 43,9 (2021): 1921-1932. doi: https://doi.org/10.1007/s10529-021-03167-1
Xiang M, Wang L, Yan Q, Jiang Z, Yang S. Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making. Biotechnol Lett. 2021 Sep;43(9):1921-1932. doi: 10.1007/s10529-021-03167-1. Epub 2021 Jul 24. PMID: 34302564.
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Biotechnol Lett. 2021 Sep;43(9):1921-1932. doi: 10.1007/s10529-021-03167-1. Epub 2021 Jul 24.

Heterologous expression and biochemical characterization of a cold-active lipase from Rhizopus microsporus suitable for oleate synthesis and bread making.

Biotechnology letters

Man Xiang, Ling Wang, Qiaojuan Yan, Zhengqiang Jiang, Shaoqing Yang

Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
  2. College of Engineering, China Agricultural University, Beijing, 100083, China.
  3. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. [email protected].

PMID: 34302564 DOI: 10.1007/s10529-021-03167-1

Abstract

OBJECTIVES: Cold-active lipases which show high specific activity at low temperatures are attractive in industrial applications in terms of product stability and energy saving. We aimed to identify novel cold-active lipase suitable for oleates synthesis and bread making.

RESULTS: A novel lipase gene (RmLipA) from Rhizopus microsporus was cloned and heterologously expressed in Pichia pastoris. The encoding sequence displayed 75% identity to the lipase from R. niveus. The highest extracellular lipase activity of 7931 U/mL was achieved in a 5-L fermentation. The recombinant enzyme (RmLipA) was optimally active at pH 8.0 and 20-25 °C, respectively, and stable over a wide pH range of 2.0-11.0. The enzyme was a cold-active lipase, exhibiting > 80% of its maximal activity at 0 °C. RmLipA was a sn-1,3 regioselective lipase, and preferred to hydrolyze pNP esters and triglycerides with relatively long chain fatty acids. RmLipA synthesized various oleates using oleic acid and different alcohols as substrates (> 95%). Moreover, it significantly improved the quality of bread by increasing its specific volume (21.7%) and decreasing its crumb firmness (28.6%).

CONCLUSIONS: A novel cold-active lipase gene from R. microsporus was identified, and its application potentials were evaluated. RmLipA should be a potential candidate in oleates synthesis and bread making industries.

© 2021. The Author(s), under exclusive licence to Springer Nature B.V.

Keywords: Cold-active lipase; High-level expression; Oleate synthesis; Rhizopus microsporus

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