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Wang J, Ma Y, Li J, et al. Effective and transient mapping of protein-protein interactions: Application of novel releasable photoaffinity linkers. Drug Dev Res. 2021;doi: 10.1002/ddr.21866.
Wang, J., Ma, Y., Li, J., Zhang, Q., Pan, X., Lu, W., & Zhang, J. (2021). Effective and transient mapping of protein-protein interactions: Application of novel releasable photoaffinity linkers. Drug development research, . https://doi.org/10.1002/ddr.21866
Wang, Jin, et al. "Effective and transient mapping of protein-protein interactions: Application of novel releasable photoaffinity linkers." Drug development research vol. (2021). doi: https://doi.org/10.1002/ddr.21866
Wang J, Ma Y, Li J, Zhang Q, Pan X, Lu W, Zhang J. Effective and transient mapping of protein-protein interactions: Application of novel releasable photoaffinity linkers. Drug Dev Res. 2021 Aug 23; doi: 10.1002/ddr.21866. Epub 2021 Aug 23. PMID: 34424555.
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Drug Dev Res. 2021 Aug 23; doi: 10.1002/ddr.21866. Epub 2021 Aug 23.

Effective and transient mapping of protein-protein interactions: Application of novel releasable photoaffinity linkers.

Drug development research

Jin Wang, Yuexiang Ma, Jing Li, Qingqing Zhang, Xiaoyan Pan, Wen Lu, Jie Zhang

Affiliations

  1. School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.

PMID: 34424555 DOI: 10.1002/ddr.21866

Abstract

Herein, two novel multifunctional releasable photoaffinity linkers were developed for effective and transient tracking interacting proteins with the overall objective of understanding their in vivo biological functions in real-time. These linkers could be used for the chemical modification of protein under moderate experimental conditions to form protein photoaffinity probes. These probes incorporated with both photoaffinity labels and tag-transfer, enable photo-crosslinking of bait proteins along with the release of unrelated groups. These photoaffinity linkers can be utilized to construct probes for disease markers, which could enable rapid diagnosis in a clinical setting at minimal interference with normal physiology.

© 2021 Wiley Periodicals LLC.

Keywords: effective and transient mapping; protein-protein interactions; releasable photoaffinity linkers

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