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Yang K, Li SG, Zhang TY, et al. Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression. J Tradit Chin Med. 2021;41(4):581-587doi: 10.19852/j.cnki.jtcm.2021.03.010.
Yang, K., Li, S. G., Zhang, T. Y., Dong, P. P., & Zeng, Q. Q. (2021). Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 41(4), 581-587. https://doi.org/10.19852/j.cnki.jtcm.2021.03.010
Yang, Kai, et al. "Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression." Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan vol. 41,4 (2021): 581-587. doi: https://doi.org/10.19852/j.cnki.jtcm.2021.03.010
Yang K, Li SG, Zhang TY, Dong PP, Zeng QQ. Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression. J Tradit Chin Med. 2021 Aug;41(4):581-587. doi: 10.19852/j.cnki.jtcm.2021.03.010. PMID: 34392651.
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J Tradit Chin Med. 2021 Aug;41(4):581-587. doi: 10.19852/j.cnki.jtcm.2021.03.010.

Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan

Kai Yang, Shu-Gen Li, Tian-Yu Zhang, Pan-Pan Dong, Qing-Qi Zeng

Affiliations

  1. Department of Andrology, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang 215600, China.
  2. Department of Urology, Suzhou Municipal Hospital, Suzhou 215002, China.
  3. Department of Andrology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
  4. Department of Andrology,Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
  5. Department of Andrology,The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.

PMID: 34392651 DOI: 10.19852/j.cnki.jtcm.2021.03.010

Abstract

OBJECTIVE: To investigate the efficacy of Runjing (RJ) extract on oligoasthenoteratozoospermia (OAT) induced by ornidazole (ORN) in rats, and to study the underlying mechanism.

METHODS: Twenty-four adult male Sprague-Dawley rats were treated with normal saline (control), ORN (OAT model), ORN + 4.725 g·kg－1·d－1 RJ extract (low-dose) and ORN+ 18.9 g·kg－1·d－1 RJ extract (high-dose) for 4 weeks. The rats were then euthanized and sperm and testis samples were collected for analysis. Sperm count, motility and morphology were calculated by sperm suspension from cauda epididymis. Testicular histopathological changes were analyzed by hematoxylin and eosin staining and TdT mediated dUTP nick end labelling. Moreover, the expression of vimentin and extracellular signal-regulated kinase (ERK) were examined through Western blot, and the distribution of vimentin was detected via immunohistochemistry.

RESULTS: ORN successfully induces seminiferous epithelium injury, cellular apoptosis, and finally OAT (P < 0.05). However, both low-dose and highdose RJ extract partially rescues the phenotypes (P < 0.05). Moreover, the expressions of vimentin and ERK were significantly altered in ORN testes (all P < 0.001), while RJ extract partially reversed these effects (P < 0.01 or P < 0.001).

CONCLUSION: RJ extract can help maintain spermatogenesis through ERK signalling, and regulating vimentin expression.

Keywords: Extracellular signal-regulated MAP kinase; Oligospermia; Runjing; Spermatogenesi; Vimentin

Publication Types

Grant support