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mBio. 2021 Aug 31;12(4):e0169621. doi: 10.1128/mBio.01696-21. Epub 2021 Aug 17.

Reconstitution of Bam Complex-Mediated Assembly of a Trimeric Porin into Proteoliposomes.

mBio

Sunyia Hussain, Janine H Peterson, Harris D Bernstein

Affiliations

  1. Genetics and Biochemistry Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Healthgrid.94365.3d, Bethesda, Maryland, USA.

PMID: 34399610 PMCID: PMC8406188 DOI: 10.1128/mBio.01696-21

Abstract

Many integral membrane proteins form oligomeric complexes, but the assembly of these structures is poorly understood. Here, we show that the assembly of OmpC, a trimeric porin that resides in the Escherichia coli outer membrane (OM), can be reconstituted

Keywords: lipopolysaccharide; membrane proteins; outer membrane; protein folding; β barrel

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