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MethodsX. 2021 May 15;8:101389. doi: 10.1016/j.mex.2021.101389. eCollection 2021.

A fast and simple ion-pair high performance liquid chromatography method for analysis of primary bile salts in .

MethodsX

Tiantian Lin, Sean O'Keefe, Cristina Fernández-Fraguas

Affiliations

  1. Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
  2. Macromolecules Innovation Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

PMID: 34430285 PMCID: PMC8374444 DOI: 10.1016/j.mex.2021.101389

Abstract

Bile salts (BS) play a key role in cholesterol and lipid metabolism as well as in many other key metabolic pathways. High performance liquid chromatography (HPLC) is the most common technique used to analyze BS in diverse type of samples. However, current HPLC analysis methods used to analyze and quantify single BS in

© 2021 The Authors. Published by Elsevier B.V.

Keywords: Bile salts; Chyme samples; HPLC; In vitro digestion; Ion-pair reagent

Conflict of interest statement

The authors confirm that there are no conflicts of interest.

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