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Wise SA, Camara JE, Burdette CQ, et al. Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D. Anal Bioanal Chem. 2021;doi: 10.1007/s00216-021-03576-1.
Wise, S. A., Camara, J. E., Burdette, C. Q., Hahm, G., Nalin, F., Kuszak, A. J., Merkel, J., Durazo-Arvizu, R. A., Williams, E. L., Hoofnagle, A. N., Ivison, F., Fischer, R., van den Ouweland, J. M. W., Ho, C. S., Law, E. W. K., Simard, J. N., Gonthier, R., Holmquist, B., Meadows, S., Cox, L., Robyak, K., Creer, M. H., Fitzgerald, R., Clarke, M. W., Breen, N., Lukas, P., Cavalier, �. �., & Sempos, C. T. (2021). Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D. Analytical and bioanalytical chemistry, . https://doi.org/10.1007/s00216-021-03576-1
Wise, Stephen A, et al. "Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D." Analytical and bioanalytical chemistry vol. (2021). doi: https://doi.org/10.1007/s00216-021-03576-1
Wise SA, Camara JE, Burdette CQ, Hahm G, Nalin F, Kuszak AJ, Merkel J, Durazo-Arvizu RA, Williams EL, Hoofnagle AN, Ivison F, Fischer R, van den Ouweland JMW, Ho CS, Law EWK, Simard JN, Gonthier R, Holmquist B, Meadows S, Cox L, Robyak K, Creer MH, Fitzgerald R, Clarke MW, Breen N, Lukas P, Cavalier É, Sempos CT. Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D. Anal Bioanal Chem. 2021 Aug 25; doi: 10.1007/s00216-021-03576-1. Epub 2021 Aug 25. PMID: 34432104.
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Anal Bioanal Chem. 2021 Aug 25; doi: 10.1007/s00216-021-03576-1. Epub 2021 Aug 25.

Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D.

Analytical and bioanalytical chemistry

Stephen A Wise, Johanna E Camara, Carolyn Q Burdette, Grace Hahm, Federica Nalin, Adam J Kuszak, Joyce Merkel, Ramón A Durazo-Arvizu, Emma L Williams, Andrew N Hoofnagle, Fiona Ivison, Ralf Fischer, Jody M W van den Ouweland, Chung S Ho, Emmett W K Law, Jean-Nicolas Simard, Renaud Gonthier, Brett Holmquist, Sarah Meadows, Lorna Cox, Kimberly Robyak, Michael H Creer, Robert Fitzgerald, Michael W Clarke, Norma Breen, Pierre Lukas, Étienne Cavalier, Christopher T Sempos

Affiliations

  1. Office of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD, 20892, USA. [email protected].
  2. Scientist Emeritus, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA. [email protected].
  3. Chemical Sciences Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD, 20899, USA.
  4. Office of Dietary Supplements (ODS), National Institutes of Health (NIH), Bethesda, MD, 20892, USA.
  5. Biostatistics Core, The Sabin Research Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90027, USA.
  6. Imperial Healthcare NHS Trust, London, W6 8RF, UK.
  7. Department of Laboratory Medicine, University of Washington, Seattle, WA, 98185, USA.
  8. Central Manchester Foundation Trust, Manchester, M13 9WL, UK.
  9. Chromsystems Instruments & Chemicals GmbH, 82166, Gräfelfing, Germany.
  10. Canisius Wilhelmina Hospital (CWZ), 6532, SZ, Nijmegen, The Netherlands.
  11. Biochemical Mass Spectrometry Unit, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, Hong Kong.
  12. Endoceutics Inc., Québec, G1P 4P5, Canada.
  13. Endocrine Sciences, LabCorp Specialty Testing Group, Agoura Hills, CA, 91301, USA.
  14. Medical Research Council (MRC) Elsie Widdowson Laboratory (closed Dec. 2018), Cambridge, UK.
  15. NIHR BRC Nutritional Biomarker Laboratory, MRC Epidemiology Unit, University of Cambridge, Cambridge, CB2 0SL, UK.
  16. College of Medicine, Penn State University, Hershey, PA, 17033, USA.
  17. Health Clinical Laboratories, University of California at San Diego, La Jolla, CA, 92093, USA.
  18. Metabolomics Australia, Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA, 6009, Australia.
  19. Waters Technologies Ireland Ltd., Wexford, Y35 D431, Ireland.
  20. Clinical Chemistry, University of Liège, CHU de Liège, B-4000, Liège, Belgium.
  21. Vitamin D Standardization Program LLC, Havre de Grace, MD, 217078, USA.

PMID: 34432104 DOI: 10.1007/s00216-021-03576-1

Abstract

An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays used for the determination of serum total 25-hydroxyvitamin D (25(OH)D), which is the sum of 25-hydroxyvitamin D

© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Keywords: 24R,25-Dihydroxyvitamin D3 (24R,25(OH)3D3); 25-Hydroxyvitamin D2 (25(OH)D2); 25-Hydroxyvitamin D3 (25(OH)D3); 3-epi-25-Hydroxyvitamin D3 (3-epi-25(OH)D3); Liquid chromatography – tandem mass spectrometry (LC-MS/MS); Total serum 25-hydroxyvitamin D (25(OH)D)

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