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Tarhan Ç, Çakır Ö. Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis. Genet Mol Biol. 2021;44(3):e20200245doi: 10.1590/1678-4685-GMB-2020-0245.
Tarhan, �. �., & Çakır, �. �. (2021). Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis. Genetics and molecular biology, 44(3), e20200245. https://doi.org/10.1590/1678-4685-GMB-2020-0245
Tarhan, Çağatay, and Çakır, Özgür. "Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis." Genetics and molecular biology vol. 44,3 (2021): e20200245. doi: https://doi.org/10.1590/1678-4685-GMB-2020-0245
Tarhan Ç, Çakır Ö. Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis. Genet Mol Biol. 2021 Aug 27;44(3):e20200245. doi: 10.1590/1678-4685-GMB-2020-0245. eCollection 2021. PMID: 34460892; PMCID: PMC8404550.
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Genet Mol Biol. 2021 Aug 27;44(3):e20200245. doi: 10.1590/1678-4685-GMB-2020-0245. eCollection 2021.

Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis.

Genetics and molecular biology

Çağatay Tarhan, Özgür Çakır

Affiliations

  1. Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul, Turkey.

PMID: 34460892 PMCID: PMC8404550 DOI: 10.1590/1678-4685-GMB-2020-0245

Abstract

While calorie restriction is the most used experimental intervention to increase lifespan in numerous model organisms, increasing evidence suggests that excess glucose leads to decreased lifespan in various organisms. To fully understand the molecular basis of the pro-aging effect of glucose, it is still important to discover genetic interactions, gene expression patterns, and molecular responses depending on glucose availability. Here, we compared the gene expression profiles in Schizosaccharomyces pombe mid-log-phase cells grown in three different Synthetic Dextrose media with 3%, 5%, and 8% glucose, using the RNA sequencing method. Expression patterns of genes that function in carbohydrate metabolism were downregulated as expected, and these genes were downregulated in line with the increase in glucose content. Significant and consistent changes in the expression were observed such as genes that encoding retrotransposable elements, heat shock proteins, glutathione S-transferase, cell agglutination protein, and conserved fungal proteins. We group some genes that function together in the transcription process and mitotic regulation, which have recently been associated with glucose availability. Our results shed light on the relationship between excess glucose, diverse cellular processes, and aging.

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