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Arulkandarajah KH, Osterstock G, Lafont A, et al. Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord. Curr Biol. 2021;31(20):4584-4595.e4doi: 10.1016/j.cub.2021.08.019.
Arulkandarajah, K. H., Osterstock, G., Lafont, A., Le Corronc, H., Escalas, N., Corsini, S., Le Bras, B., Chenane, L., Boeri, J., Czarnecki, A., Mouffle, C., Bullier, E., Hong, E., Soula, C., Legendre, P., & Mangin, J. M. (2021). Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord. Current biology : CB, 31(20), 4584-4595.e4. https://doi.org/10.1016/j.cub.2021.08.019
Arulkandarajah, Kalaimakan Hervé, et al. "Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord." Current biology : CB vol. 31,20 (2021): 4584-4595.e4. doi: https://doi.org/10.1016/j.cub.2021.08.019
Arulkandarajah KH, Osterstock G, Lafont A, Le Corronc H, Escalas N, Corsini S, Le Bras B, Chenane L, Boeri J, Czarnecki A, Mouffle C, Bullier E, Hong E, Soula C, Legendre P, Mangin JM. Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord. Curr Biol. 2021 Oct 25;31(20):4584-4595.e4. doi: 10.1016/j.cub.2021.08.019. Epub 2021 Sep 02. PMID: 34478646.
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Curr Biol. 2021 Oct 25;31(20):4584-4595.e4. doi: 10.1016/j.cub.2021.08.019. Epub 2021 Sep 02.

Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord.

Current biology : CB

Kalaimakan Hervé Arulkandarajah, Guillaume Osterstock, Agathe Lafont, Hervé Le Corronc, Nathalie Escalas, Silvia Corsini, Barbara Le Bras, Linda Chenane, Juliette Boeri, Antonny Czarnecki, Christine Mouffle, Erika Bullier, Elim Hong, Cathy Soula, Pascal Legendre, Jean-Marie Mangin

Affiliations

  1. Sorbonne Université, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 75005 Paris, France.
  2. Sorbonne Université, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 75005 Paris, France; Université d'Angers, 49000 Angers, France.
  3. Centre de Biologie du Développement (CBD) CNRS/UPS, Centre de Biologie Intégrative (CBI), Université de Toulouse, 31000 Toulouse, France.
  4. Sorbonne Université, INSERM, CNRS, Neurosciences Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), 75005 Paris, France. Electronic address: [email protected].

PMID: 34478646 DOI: 10.1016/j.cub.2021.08.019

Abstract

In the developing central nervous system, electrical signaling is thought to rely exclusively on differentiating neurons as they acquire the ability to generate and propagate action potentials. Accordingly, neuroepithelial progenitors (NEPs), which give rise to all neurons and glial cells during development, have been reported to remain electrically passive. Here, we investigated the physiological properties of NEPs at the onset of spontaneous neural activity (SNA) initiating motor behavior in mouse embryonic spinal cord. Using patch-clamp recordings, we discovered that spinal NEPs exhibit spontaneous membrane depolarizations during episodes of SNA. These rhythmic depolarizations exhibited a ventral-to-dorsal gradient with the highest amplitude located in the floor plate, the ventral-most part of the neuroepithelium. Paired recordings revealed that NEPs are coupled via gap junctions and form an electrical syncytium. Although other NEPs were electrically passive, we discovered that floor-plate NEPs generated large Na

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: ATP; calcium imaging; connexins; electrophysiology; glia; motor behavior; neural stem cells; optogenetics; spinal cord; voltage-gated calcium channels

Conflict of interest statement

Declaration of interests The authors declare no competing interests.

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