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Antonsen KW, Hviid CVB, Hagensen MK, et al. Soluble PD-1 (sPD-1) is expressed in human macrophages. Cell Immunol. 2021;369:104435doi: 10.1016/j.cellimm.2021.104435.
Antonsen, K. W., Hviid, C. V. B., Hagensen, M. K., Sørensen, B. S., & Møller, H. J. (2021). Soluble PD-1 (sPD-1) is expressed in human macrophages. Cellular immunology, 369104435. https://doi.org/10.1016/j.cellimm.2021.104435
Antonsen, Kristian W, et al. "Soluble PD-1 (sPD-1) is expressed in human macrophages." Cellular immunology vol. 369 (2021): 104435. doi: https://doi.org/10.1016/j.cellimm.2021.104435
Antonsen KW, Hviid CVB, Hagensen MK, Sørensen BS, Møller HJ. Soluble PD-1 (sPD-1) is expressed in human macrophages. Cell Immunol. 2021 Nov;369:104435. doi: 10.1016/j.cellimm.2021.104435. Epub 2021 Aug 29. PMID: 34482260.
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Cell Immunol. 2021 Nov;369:104435. doi: 10.1016/j.cellimm.2021.104435. Epub 2021 Aug 29.

Soluble PD-1 (sPD-1) is expressed in human macrophages.

Cellular immunology

Kristian W Antonsen, Claus V B Hviid, Mette K Hagensen, Boe S Sørensen, Holger J Møller

Affiliations

  1. Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark.
  2. Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark. Electronic address: [email protected].

PMID: 34482260 DOI: 10.1016/j.cellimm.2021.104435

Abstract

The PD-1/PD-L1 axis plays a crucial role in regulating the anti-tumour immune response. A soluble PD-1 protein (sPD-1) has previously been observed, which could block the binding of PD-L1 to PD-1. Tumour associated macrophages are abundant in tumours, and evidence suggest they express PD-1. However, whether they also express sPD-1 remains unclear. The objective of this study was to investigate expression of sPD-1 in two in vitro models of human macrophages: THP-1 cells and monocyte-derived macrophages (MDM). Cells were polarised with either LPS + IFN-γ or IL-4 + IL-13 or left unpolarised. PD-1 and sPD-1 mRNAs were measured using droplet digital PCR, sPD-1 protein by electrochemiluminescence immunoassay and PD-1 by flow cytometry. sPD-1 mRNA was induced in both THP-1 cells and MDM after polarisation with LPS + IFN-γ, while IL-4 + IL-13 induced sPD-1 mRNA in MDM only. sPD-1 protein was measurable in culture supernatants. These findings show that macrophages can be induced to express sPD-1.

Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Keywords: Macrophages; PD-1; Soluble PD-1; Tumor associated macrophages; Tumor immunology

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