Display options
Cite
Ryabkov MG, Kiseleva EB, Baleev MS, et al. Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall. Sovrem Tekhnologii Med. 2020;12(2):56-64doi: 10.17691/stm2020.12.2.07.
Ryabkov, M. G., Kiseleva, E. B., Baleev, M. S., Bederina, E. L., Sizov, M. A., Vorobyov, A. N., Moiseev, A. A., Karabut, M. M., Plekhanova, M. A., & Gladkova, N. D. (2020). Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall. Sovremennye tekhnologii v meditsine, 12(2), 56-64. https://doi.org/10.17691/stm2020.12.2.07
Ryabkov, M G, et al. "Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall." Sovremennye tekhnologii v meditsine vol. 12,2 (2020): 56-64. doi: https://doi.org/10.17691/stm2020.12.2.07
Ryabkov MG, Kiseleva EB, Baleev MS, Bederina EL, Sizov MA, Vorobyov AN, Moiseev AA, Karabut MM, Plekhanova MA, Gladkova ND. Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall. Sovrem Tekhnologii Med. 2020;12(2):56-64. doi: 10.17691/stm2020.12.2.07. PMID: 34513054; PMCID: PMC8353680.
Copy Download .nbib Format: NLM
Share it on

Sovrem Tekhnologii Med. 2020;12(2):56-64. doi: 10.17691/stm2020.12.2.07.

Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall.

Sovremennye tekhnologii v meditsine

M G Ryabkov, E B Kiseleva, M S Baleev, E L Bederina, M A Sizov, A N Vorobyov, A A Moiseev, M M Karabut, M A Plekhanova, N D Gladkova

Affiliations

  1. Associate Professor, Leading Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
  2. Senior Researcher, Scientific Laboratory of Optical Coherence Tomography, Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
  3. Surgeon, City Clinical Hospital No.30, 85A Berezovskaya St., Nizhny Novgorod, 605157, Russia.
  4. Pathologist, Junior Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
  5. Senior Researcher, Laboratory of Highly Sensitive Optical Measurements, Federal Research Center Institute of Applied Physics of the Russian Academy of Sciences, 46 Ul'yanova St., Nizhny Novgorod, 603950, Russia.
  6. Researcher, Genomics Adaptive Antitumor Immunity Research Laboratory, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
  7. Student, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
  8. Professor, Head of the Scientific Laboratory of Optical Coherence Tomography, Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.

PMID: 34513054 PMCID: PMC8353680 DOI: 10.17691/stm2020.12.2.07

Abstract

MATERIALS AND METHODS: In experiments on Wistar rats (n=22), we examined the small intestine wall

RESULTS: Trans-serous MM OCT allowed us to visualize the bowel wall to its entire thickness, distinguish between the serous-muscular and mucous-submucosal layers, and detect the villi and functioning blood vessels. The structures were best seen after a fat emulsion had been administered into the bowel lumen. In OCT images made in the optical coherent angiography (OCA) mode, large paired vessels (arteries and veins) and micro-vessels with a diameter of >15 μm could be seen. Most of the blood vessels were imaged in the depth range of 80-300 μm from the surface. Capillaries with a diameter of 7-10 μm were not seen, but they produced an overall bright background. In the OCA images reconstructed from a volume of 2.4×2.4×1.8 mm, the total length of the vascular bed before ischemia was 18.3 [16.6; 19.8] mm.Strangulation of the intestinal loop was associated with changes in the CP OCT picture: the villi-associated vertical pattern and shadows of blood vessels disappeared and the depth of tissue visualization in the cross-channel decreased. The optical equivalents of the serous-muscular layer were preserved; after 180±12 min of ischemia, their proportion in the intestinal wall thickness increased from 25 [18; 32] to 42 [31; 55]% (p=0.031). At that time-point, OCA images of the strangulated bowel loop looked all similar: a uniform dark background with isolated fragmentary large vessels and no signs of blood flow in the microvascular network.

CONCLUSION: Trans-serous MM OCT provides for

Keywords: CP OCT; cross-polarization optical coherence tomography; histomorphometry; optical coherence angiography; small bowel ischemia; small intestinal strangulation; viability of the small intestine

Conflict of interest statement

Conflict of interests. The authors have no conflict of interest.

References

  1. BMC Surg. 2013;13 Suppl 2:S51 - PubMed
  2. J Gastroenterol Hepatol. 2007 Dec;22(12):2256-60 - PubMed
  3. Gastrointest Endosc. 2003 Aug;58(2):196-202 - PubMed
  4. Int J Biomed Imaging. 2012;2012:940585 - PubMed
  5. Endoscopy. 2000 Oct;32(10):750-5 - PubMed
  6. World J Gastrointest Surg. 2011 May 27;3(5):59-69 - PubMed
  7. Am J Surg. 2020 Aug;220(2):309-315 - PubMed
  8. Biomed Opt Express. 2014 Nov 24;5(12):4387-404 - PubMed
  9. Dig Liver Dis. 2009 Sep;41(9):639-43 - PubMed
  10. J Androl. 2012 May-Jun;33(3):499-504 - PubMed
  11. Cells Tissues Organs. 2017;203(6):353-364 - PubMed
  12. Surg Endosc. 2017 Mar;31(3):1061-1069 - PubMed
  13. J Visc Surg. 2015 Feb;152(1):71-2 - PubMed
  14. Opt Express. 1997 Dec 22;1(13):432-40 - PubMed
  15. Colorectal Dis. 2016 Mar;18(3):O103-10 - PubMed
  16. Am J Gastroenterol. 2001 Sep;96(9):2633-9 - PubMed
  17. Surg Endosc. 2018 Oct;32(10):4351-4356 - PubMed
  18. Ther Adv Chronic Dis. 2014 Jan;5(1):15-29 - PubMed
  19. J Biomed Opt. 2014;19(7):071412 - PubMed
  20. Biomed Opt Express. 2017 Apr 07;8(5):2405-2444 - PubMed
  21. Sci Rep. 2017 Nov 28;7(1):16505 - PubMed
  22. J Biomed Opt. 2017 Dec;22(12):1-17 - PubMed
  23. J Biophotonics. 2018 Oct;11(10):e201700292 - PubMed
  24. J Gastroenterol. 1997 Aug;32(4):457-63 - PubMed

Publication Types