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Sandberg MW, Bunkenborg J, Thyssen S, et al. Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry. Amino Acids. 2021;doi: 10.1007/s00726-021-03004-9.
Sandberg, M. W., Bunkenborg, J., Thyssen, S., Villadsen, M., & Kofoed, T. (2021). Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry. Amino acids, . https://doi.org/10.1007/s00726-021-03004-9
Sandberg, Magdalena Widgren, et al. "Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry." Amino acids vol. (2021). doi: https://doi.org/10.1007/s00726-021-03004-9
Sandberg MW, Bunkenborg J, Thyssen S, Villadsen M, Kofoed T. Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry. Amino Acids. 2021 Aug 28; doi: 10.1007/s00726-021-03004-9. Epub 2021 Aug 28. PMID: 34453584.
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Amino Acids. 2021 Aug 28; doi: 10.1007/s00726-021-03004-9. Epub 2021 Aug 28.

Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry.

Amino acids

Magdalena Widgren Sandberg, Jakob Bunkenborg, Stine Thyssen, Martin Villadsen, Thomas Kofoed

Affiliations

  1. Universitätsklinikum Hamburg-Eppendorf (UKE), Hamburg, Germany. [email protected].
  2. Alphalyse A/S, Odense, Denmark. [email protected].
  3. Alphalyse A/S, Odense, Denmark.

PMID: 34453584 DOI: 10.1007/s00726-021-03004-9

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine and a white blood cell growth factor that has found usage as a therapeutic protein. During analysis of different fermentation batches of GM-CSF recombinantly expressed in E. coli, a covalent modification was identified on the protein by intact mass spectrometry. The modification gave a mass shift of + 70 Da and peptide mapping analysis demonstrated that it located to the protein N-terminus and lysine side chains. The chemical composition of C

© 2021. The Author(s).

Keywords: Crotonaldehyde; E. coli; Granulocyte-macrophage colony-stimulating factor; Post-translation modification; Recombinant protein production

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