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Costa C, Semedo M, Machado SP, et al. Transcriptional analyses reveal different mechanism of toxicity for a chronic exposure to fluoxetine and venlafaxine on the brain of the marine fish Dicentrarchrus labrax. Comp Biochem Physiol C Toxicol Pharmacol. 2021;250:109170doi: 10.1016/j.cbpc.2021.109170.
Costa, C., Semedo, M., Machado, S. P., Cunha, V., Ferreira, M., & Urbatzka, R. (2021). Transcriptional analyses reveal different mechanism of toxicity for a chronic exposure to fluoxetine and venlafaxine on the brain of the marine fish Dicentrarchrus labrax. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 250109170. https://doi.org/10.1016/j.cbpc.2021.109170
Costa, Carolina, et al. "Transcriptional analyses reveal different mechanism of toxicity for a chronic exposure to fluoxetine and venlafaxine on the brain of the marine fish Dicentrarchrus labrax." Comparative biochemistry and physiology. Toxicology & pharmacology : CBP vol. 250 (2021): 109170. doi: https://doi.org/10.1016/j.cbpc.2021.109170
Costa C, Semedo M, Machado SP, Cunha V, Ferreira M, Urbatzka R. Transcriptional analyses reveal different mechanism of toxicity for a chronic exposure to fluoxetine and venlafaxine on the brain of the marine fish Dicentrarchrus labrax. Comp Biochem Physiol C Toxicol Pharmacol. 2021 Dec;250:109170. doi: 10.1016/j.cbpc.2021.109170. Epub 2021 Aug 25. PMID: 34454087.
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Comp Biochem Physiol C Toxicol Pharmacol. 2021 Dec;250:109170. doi: 10.1016/j.cbpc.2021.109170. Epub 2021 Aug 25.

Transcriptional analyses reveal different mechanism of toxicity for a chronic exposure to fluoxetine and venlafaxine on the brain of the marine fish Dicentrarchrus labrax.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Carolina Costa, Miguel Semedo, Sandrine P Machado, Virginia Cunha, Marta Ferreira, Ralph Urbatzka

Affiliations

  1. Interdisciplinary Center for Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Matosinhos, Portugal.
  2. Interdisciplinary Center for Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Matosinhos, Portugal; School of Marine Studies, Faculty of Science, Technology & Environment, University of South Pacific, Laucala Bay Road, Suva, Fiji.
  3. Interdisciplinary Center for Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Matosinhos, Portugal. Electronic address: [email protected].

PMID: 34454087 DOI: 10.1016/j.cbpc.2021.109170

Abstract

Selective serotonin reuptake inhibitor (SSRI) and serotonin norepinephrine reuptake inhibitor (SNRI) are prescribed for clinical depression and detected in aquatic ecosystems. The main aim of this study was to explore and evaluate transcriptional responses of neurotransmitter genes in the brain of a marine fish species, European seabass, and to analyze global brain transcriptomic changes by a RNA-seq technology (MACE, massive analysis of cDNA ends). The juveniles were exposed to two psychopharmaceuticals: (i) fluoxetine (FLX) at the concentration of 0.5 μg/L and 50 μg/L; (ii) venlafaxine (VENX) at the concentration of 0.01 μg/L and 1 μg/L. The exposures were performed for 21 days, followed by a 7-day recovery period to assess the reversibility of effects. Both psychopharmaceuticals affected differentially the neurotransmitter mRNA expression analyzed by RT-qPCR (serotonin receptors: 5-ht3a, 5-ht3b; dopamine receptors: d2, d3; neurotransmitter transporter: sert, vmat; degrading enzyme: mao). Transcriptomic analyses after 21 days of exposure revealed 689 and 632 significant different transcripts by FLX at 0.5 and 50 μg/L, respectively, and 432 and 1250 by VENX at 0.01 and 1 μg/L, respectively, and confirmed different mechanism of toxicity between both compounds. At environmental concentrations, more general pathways including energy metabolism were affected, while at the higher concentration effects on neurotransmitter pathways were observed (FLX: exocytosis and vesicle formation; VENX: small molecule catabolism regulating dopamine and tyrosine level). These results provided new insights into the chronic effects of psychopharmaceutical compounds on marine fish and suggest the need of a separate ecotoxicological risk analysis.

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: European seabass; Neurotransmitter; Psychopharmaceutical compounds; Risk assessment; Transcriptomics

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