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Murakawa T, Suzuki M, Arima T, et al. Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase. Acta Crystallogr F Struct Biol Commun. 2021;77:356-363doi: 10.1107/S2053230X21008967.
Murakawa, T., Suzuki, M., Arima, T., Sugahara, M., Tanaka, T., Tanaka, R., Iwata, S., Nango, E., Tono, K., Hayashi, H., Fukui, K., Yano, T., Tanizawa, K., & Okajima, T. (2021). Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase. Acta crystallographica. Section F, Structural biology communications, 77356-363. https://doi.org/10.1107/S2053230X21008967
Murakawa, Takeshi, et al. "Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase." Acta crystallographica. Section F, Structural biology communications vol. 77 (2021): 356-363. doi: https://doi.org/10.1107/S2053230X21008967
Murakawa T, Suzuki M, Arima T, Sugahara M, Tanaka T, Tanaka R, Iwata S, Nango E, Tono K, Hayashi H, Fukui K, Yano T, Tanizawa K, Okajima T. Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase. Acta Crystallogr F Struct Biol Commun. 2021 Oct 01;77:356-363. doi: 10.1107/S2053230X21008967. Epub 2021 Sep 21. PMID: 34605440; PMCID: PMC8488853.
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Acta Crystallogr F Struct Biol Commun. 2021 Oct 01;77:356-363. doi: 10.1107/S2053230X21008967. Epub 2021 Sep 21.

Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase.

Acta crystallographica. Section F, Structural biology communications

Takeshi Murakawa, Mamoru Suzuki, Toshi Arima, Michihiro Sugahara, Tomoyuki Tanaka, Rie Tanaka, So Iwata, Eriko Nango, Kensuke Tono, Hideyuki Hayashi, Kenji Fukui, Takato Yano, Katsuyuki Tanizawa, Toshihide Okajima

Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
  2. Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
  3. SPring-8 Center, RIKEN, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
  4. Department of Chemistry, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
  5. Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

PMID: 34605440 PMCID: PMC8488853 DOI: 10.1107/S2053230X21008967

Abstract

Recent advances in serial femtosecond X-ray crystallography (SFX) using X-ray free-electron lasers have paved the way for determining radiation-damage-free protein structures under nonfreezing conditions. However, the large-scale preparation of high-quality microcrystals of uniform size is a prerequisite for SFX, and this has been a barrier to its widespread application. Here, a convenient method for preparing high-quality microcrystals of a bacterial quinoprotein enzyme, copper amine oxidase from Arthrobacter globiformis, is reported. The method consists of the mechanical crushing of large crystals (5-15 mm

Keywords: X-ray free-electron lasers; copper amine oxidase; microcrystals; radiation-damage-free protein structure; serial femtosecond X-ray crystallography

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