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Palombella S, Salehi S, Mondadori C, et al. Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation. Methods Mol Biol. 2022;2373:253-266doi: 10.1007/978-1-0716-1693-2_15.
Palombella, S., Salehi, S., Mondadori, C., Talò, G., Sansone, V., Moretti, M., & Lopa, S. (2022). Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation. Methods in molecular biology (Clifton, N.J.), 2373253-266. https://doi.org/10.1007/978-1-0716-1693-2_15
Palombella, Silvia, et al. "Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation." Methods in molecular biology (Clifton, N.J.) vol. 2373 (2022): 253-266. doi: https://doi.org/10.1007/978-1-0716-1693-2_15
Palombella S, Salehi S, Mondadori C, Talò G, Sansone V, Moretti M, Lopa S. Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation. Methods Mol Biol. 2022;2373:253-266. doi: 10.1007/978-1-0716-1693-2_15. PMID: 34520017.
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Methods Mol Biol. 2022;2373:253-266. doi: 10.1007/978-1-0716-1693-2_15.

Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation.

Methods in molecular biology (Clifton, N.J.)

Silvia Palombella, Shima Salehi, Carlotta Mondadori, Giuseppe Talò, Valerio Sansone, Matteo Moretti, Silvia Lopa

Affiliations

  1. IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue Engineering Laboratory, Milan, Italy.
  2. IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
  3. Regenerative Medicine Technologies Lab, Ente Ospedaliero Cantonale, Lugano, Switzerland.
  4. Euler Institute, Faculty of Biomedical Sciences, Università della Svizzera Italiana, Lugano, Switzerland.
  5. IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue Engineering Laboratory, Milan, Italy. [email protected].

PMID: 34520017 DOI: 10.1007/978-1-0716-1693-2_15

Abstract

Microfluidics allows for recapitulating organotypic environments in miniaturized cell culture platforms. This ability paves the way to the investigation of complex biological processes in a relevant milieu. Here we describe the protocols to generate an organotypic model including a vascularized compartment mimicking the synovial membrane and designed for the study of monocyte extravasation during osteoarthritis.

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: Cartilage; Extravasation; Microfluidics; Monocyte; Organ-on-a-chip; Synovium

References

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