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Kremsmayr T, Muttenthaler M. Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues. Methods Mol Biol. 2022;2384:175-199doi: 10.1007/978-1-0716-1759-5_11.
Kremsmayr, T., & Muttenthaler, M. (2022). Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues. Methods in molecular biology (Clifton, N.J.), 2384175-199. https://doi.org/10.1007/978-1-0716-1759-5_11
Kremsmayr, Thomas, and Muttenthaler, Markus. "Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues." Methods in molecular biology (Clifton, N.J.) vol. 2384 (2022): 175-199. doi: https://doi.org/10.1007/978-1-0716-1759-5_11
Kremsmayr T, Muttenthaler M. Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues. Methods Mol Biol. 2022;2384:175-199. doi: 10.1007/978-1-0716-1759-5_11. PMID: 34550575.
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Methods Mol Biol. 2022;2384:175-199. doi: 10.1007/978-1-0716-1759-5_11.

Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues.

Methods in molecular biology (Clifton, N.J.)

Thomas Kremsmayr, Markus Muttenthaler

Affiliations

  1. Faculty of Chemistry, Institute of Biological Chemistry, University of Vienna, Vienna, Austria.
  2. Faculty of Chemistry, Institute of Biological Chemistry, University of Vienna, Vienna, Austria. [email protected].
  3. Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia. [email protected].

PMID: 34550575 DOI: 10.1007/978-1-0716-1759-5_11

Abstract

Solid phase peptide synthesis is the most commonly used method for the production of peptides. In this chapter, we outline the standard operating procedures used in our laboratory to efficiently access oxytocin-like peptides. This includes detailed descriptions of equipment setup, reagent selection, peptide assembly on solid support, peptide side chain deprotection and cleavage from the solid support, oxidative folding, purification, and analysis.

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: 9-fluorenylmethoxycarbonyl (Fmoc); Amino acid coupling; Fmoc-SPPS; Oxidative peptide folding; Solid phase peptide synthesis (SPPS); Synthetic oxytocin analogues

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