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Głowacz K, Drozd M, Ciosek-Skibińska P. Excitation-emission fluorescence matrix acquired from glutathione capped CdSeS/ZnS quantum dots in combination with chemometric tools for pattern-based sensing of neurotransmitters. Mikrochim Acta. 2021;188(10):343doi: 10.1007/s00604-021-04984-x.
Głowacz, K., Drozd, M., & Ciosek-Skibińska, P. (2021). Excitation-emission fluorescence matrix acquired from glutathione capped CdSeS/ZnS quantum dots in combination with chemometric tools for pattern-based sensing of neurotransmitters. Mikrochimica acta, 188(10), 343. https://doi.org/10.1007/s00604-021-04984-x
Głowacz, Klaudia, et al. "Excitation-emission fluorescence matrix acquired from glutathione capped CdSeS/ZnS quantum dots in combination with chemometric tools for pattern-based sensing of neurotransmitters." Mikrochimica acta vol. 188,10 (2021): 343. doi: https://doi.org/10.1007/s00604-021-04984-x
Głowacz K, Drozd M, Ciosek-Skibińska P. Excitation-emission fluorescence matrix acquired from glutathione capped CdSeS/ZnS quantum dots in combination with chemometric tools for pattern-based sensing of neurotransmitters. Mikrochim Acta. 2021 Sep 15;188(10):343. doi: 10.1007/s00604-021-04984-x. PMID: 34524529; PMCID: PMC8443496.
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Mikrochim Acta. 2021 Sep 15;188(10):343. doi: 10.1007/s00604-021-04984-x.

Excitation-emission fluorescence matrix acquired from glutathione capped CdSeS/ZnS quantum dots in combination with chemometric tools for pattern-based sensing of neurotransmitters.

Mikrochimica acta

Klaudia Głowacz, Marcin Drozd, Patrycja Ciosek-Skibińska

Affiliations

  1. Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland. [email protected].
  2. Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland.
  3. Centre for Advanced Materials and Technologies CEZAMAT, Poleczki 19, 02-822, Warsaw, Poland.
  4. Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland. [email protected].

PMID: 34524529 PMCID: PMC8443496 DOI: 10.1007/s00604-021-04984-x

Abstract

The presented work concerns pattern-based sensing with quantum dots for the identification and quantification of neurotransmitters by means of excitation-emission fluorescence spectroscopy (2D fluorescence). In the framework of this study, glutathione capped CdSeS/ZnS nanocrystals were used as non-specific nanoreceptors capable of differentiated interaction with neurotransmitters. The pattern-based sensing with QDs was realized by using excitation-emission fluorescence spectroscopy to provide analyte-specific multidimensional optical information. These characteristic fluorescent response patterns were processed by unfolded partial least squares-discriminant analysis, showing that satisfactory identification of all investigated neurotransmitters: dopamine, norepinephrine, epinephrine, serotonin, GABA, and acetylcholine, can be achieved through the proposed sensing strategy. The impact of the considered fluorescence signal (datum, i.e. zeroth-order data acquired per sample; spectrum, i.e. first-order data acquired per sample; excitation-emission matrix, i.e. second-order data acquired per sample) on the sensing capability of glutathione capped QDs was also verified. The best performance parameters such as accuracy, precision, sensitivity, and specificity were obtained using excitation-emission matrices (88.9-93.3%, 0.93-0.95, 0.89-0.93, and 0.99-1.00, respectively). Thus, it was revealed that excitation-emission fluorescence spectroscopy may improve the recognition of neurotransmitters while using only one type of nanoreceptor. Furthermore, is was demonstrated that the proposed excitation-emission fluorescence spectroscopy assisted QD assay coupled with unfolded partial least squares regression can be successfully utilized for quantitative determination of catecholamine neurotransmitters at the micromolar concentration range with R

© 2021. The Author(s).

Keywords: 2D fluorescence; Excitation-emission matrix; Neurotransmitters; Pattern-based sensing; Quantum dots

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