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Blanco-Touriñán N, Esteve-Bruna D, Serrano-Mislata A, et al. A genetic approach reveals different modes of action of prefoldins. Plant Physiol. 2021;187(3):1534-1550doi: 10.1093/plphys/kiab348.
Blanco-Touriñán, N., Esteve-Bruna, D., Serrano-Mislata, A., Esquinas-Ariza, R. M., Resentini, F., Forment, J., Carrasco-López, C., Novella-Rausell, C., Palacios-Abella, A., Carrasco, P., Salinas, J., Blázquez, M. �. �., & Alabadí, D. (2021). A genetic approach reveals different modes of action of prefoldins. Plant physiology, 187(3), 1534-1550. https://doi.org/10.1093/plphys/kiab348
Blanco-Touriñán, Noel, et al. "A genetic approach reveals different modes of action of prefoldins." Plant physiology vol. 187,3 (2021): 1534-1550. doi: https://doi.org/10.1093/plphys/kiab348
Blanco-Touriñán N, Esteve-Bruna D, Serrano-Mislata A, Esquinas-Ariza RM, Resentini F, Forment J, Carrasco-López C, Novella-Rausell C, Palacios-Abella A, Carrasco P, Salinas J, Blázquez MÁ, Alabadí D. A genetic approach reveals different modes of action of prefoldins. Plant Physiol. 2021 Nov 03;187(3):1534-1550. doi: 10.1093/plphys/kiab348. PMID: 34618031; PMCID: PMC8566299.
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Plant Physiol. 2021 Nov 03;187(3):1534-1550. doi: 10.1093/plphys/kiab348.

A genetic approach reveals different modes of action of prefoldins.

Plant physiology

Noel Blanco-Touriñán, David Esteve-Bruna, Antonio Serrano-Mislata, Rosa María Esquinas-Ariza, Francesca Resentini, Javier Forment, Cristian Carrasco-López, Claudio Novella-Rausell, Alberto Palacios-Abella, Pedro Carrasco, Julio Salinas, Miguel Á Blázquez, David Alabadí

Affiliations

  1. Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), 46022 Valencia, Spain.
  2. Dipartimento di Bioscienze, Università degli Studi di Milano, 20133 Milano, Italy.
  3. Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas Margarita Salas (CSIC), 28040 Madrid, Spain.
  4. Departament de Bioquímica i Biologia Molecular, Universitat de València, 46100 Burjassot, Spain.

PMID: 34618031 PMCID: PMC8566299 DOI: 10.1093/plphys/kiab348

Abstract

The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved in eukaryotes and is composed of subunits PFD1-6, and PFDc-TRiC/CCT folds actin and tubulins. PFDs also participate in a wide range of cellular processes, both in the cytoplasm and in the nucleus, and their malfunction causes developmental alterations and disease in animals and altered growth and environmental responses in yeast and plants. Genetic analyses in yeast indicate that not all of their functions require the canonical complex. The lack of systematic genetic analyses in plants and animals, however, makes it difficult to discern whether PFDs participate in a process as the canonical complex or in alternative configurations, which is necessary to understand their mode of action. To tackle this question, and on the premise that the canonical complex cannot be formed if one subunit is missing, we generated an Arabidopsis (Arabidopsis thaliana) mutant deficient in the six PFDs and compared various growth and environmental responses with those of the individual mutants. In this way, we demonstrate that the PFDc is required for seed germination, to delay flowering, or to respond to high salt stress or low temperature, whereas at least two PFDs redundantly attenuate the response to osmotic stress. A coexpression analysis of differentially expressed genes in the sextuple mutant identified several transcription factors, including ABA INSENSITIVE 5 (ABI5) and PHYTOCHROME-INTERACTING FACTOR 4, acting downstream of PFDs. Furthermore, the transcriptomic analysis allowed assigning additional roles for PFDs, for instance, in response to higher temperature.

© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.

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