Display options
Cite
Schuster R, Simões T, Brave FD, et al. Separation and Visualization of Low Abundant Ubiquitylated Forms. Bio Protoc. 2018;8(22):e3081doi: 10.21769/BioProtoc.3081.
Schuster, R., Simões, T., Brave, F. D., & Escobar-Henriques, M. (2018). Separation and Visualization of Low Abundant Ubiquitylated Forms. Bio-protocol, 8(22), e3081. https://doi.org/10.21769/BioProtoc.3081
Schuster, Ramona, et al. "Separation and Visualization of Low Abundant Ubiquitylated Forms." Bio-protocol vol. 8,22 (2018): e3081. doi: https://doi.org/10.21769/BioProtoc.3081
Schuster R, Simões T, Brave FD, Escobar-Henriques M. Separation and Visualization of Low Abundant Ubiquitylated Forms. Bio Protoc. 2018 Nov 20;8(22):e3081. doi: 10.21769/BioProtoc.3081. eCollection 2018 Nov 20. PMID: 34532539; PMCID: PMC8342087.
Copy Download .nbib Format: NLM
Share it on

Bio Protoc. 2018 Nov 20;8(22):e3081. doi: 10.21769/BioProtoc.3081. eCollection 2018 Nov 20.

Separation and Visualization of Low Abundant Ubiquitylated Forms.

Bio-protocol

Ramona Schuster, Tânia Simões, Fabian Den Brave, Mafalda Escobar-Henriques

Affiliations

  1. Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases, University of Cologne, Cologne, Germany.
  2. Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried, Germany.

PMID: 34532539 PMCID: PMC8342087 DOI: 10.21769/BioProtoc.3081

Abstract

In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in

Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Keywords: Fzo1; SDS-PAGE; Western blot; immunoprecipitation; ubiquitylation

Conflict of interest statement

Competing interestsThe authors declare they have no conflict of interest or competing interests.

References

  1. J Cell Sci. 2011 Apr 1;124(Pt 7):1126-35 - PubMed
  2. Mol Cell. 2013 Feb 7;49(3):487-98 - PubMed
  3. Elife. 2018 Jan 08;7: - PubMed
  4. Yeast. 1998 Jan 30;14(2):115-32 - PubMed

Publication Types