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Yoshida A, Inaba K, Sasaki H, et al. Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution. Photodiagnosis Photodyn Ther. 2021;36:102576doi: 10.1016/j.pdpdt.2021.102576.
Yoshida, A., Inaba, K., Sasaki, H., Hamada, N., & Yoshino, F. (2021). Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution. Photodiagnosis and photodynamic therapy, 36102576. https://doi.org/10.1016/j.pdpdt.2021.102576
Yoshida, Ayaka, et al. "Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution." Photodiagnosis and photodynamic therapy vol. 36 (2021): 102576. doi: https://doi.org/10.1016/j.pdpdt.2021.102576
Yoshida A, Inaba K, Sasaki H, Hamada N, Yoshino F. Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution. Photodiagnosis Photodyn Ther. 2021 Dec;36:102576. doi: 10.1016/j.pdpdt.2021.102576. Epub 2021 Oct 07. PMID: 34628072.
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Photodiagnosis Photodyn Ther. 2021 Dec;36:102576. doi: 10.1016/j.pdpdt.2021.102576. Epub 2021 Oct 07.

Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution.

Photodiagnosis and photodynamic therapy

Ayaka Yoshida, Keitaro Inaba, Haruka Sasaki, Nobushiro Hamada, Fumihiko Yoshino

Affiliations

  1. Department of Dental Education, Kanagawa Dental University, 82 Inaoka-cho, Kanagawa, Yokosuka 238-8580, Japan.
  2. Department of Oral Microbiology, Kanagawa Dental University, 82 Inaoka-cho, Kanagawa, Yokosuka 238-8580, Japan.
  3. Kanagawa Dental University, 82 Inaoka-cho, Yokosuka 238-8580, Japan.
  4. Department of Pharmacology, Kanagawa Dental University, 82 Inaoka-cho, Kanagawa, Yokosuka 238-8580, Japan. Electronic address: [email protected].

PMID: 34628072 DOI: 10.1016/j.pdpdt.2021.102576

Abstract

OBJECTIVES: Antimicrobial photodynamic therapy (aPDT) in periodontal pockets using lasers is difficult to perform in some cases because of the high cost of irradiation equipment and the narrow irradiation field. The purpose of the present study was to examine the effects of aPDT in combination with a plaque-disclosing solution and blue light-emitting diode (LED), which are used for composite resin polymerization.

METHODS: The reactive oxygen species generated by irradiating 0.001% RB or MB with blue light were analyzed using electron spin resonance spectroscopy. Blue-light exposure was performed at 6.92, 20.76 and 124.6 J. The microorganism to be sterilized was Porphyromonas gingivalis. After aPDT, colony-forming units (CFUs) were measured to estimate cell survival. Carbonylated protein (PC) levels were used to evaluate oxidative stress. All statistical analyses were performed with Tukey's multiple comparisons test or the unpaired t-test.

RESULTS: Singlet oxygen (

CONCLUSIONS: The present results suggest that aPDT exerts bactericidal effects against P. gingivalis by increasing oxidative stress through the generation of

Copyright © 2021 Elsevier B.V. All rights reserved.

Keywords: Antimicrobial photodynamic therapy; Blue light; Oxidative stress; Plaque disclosing solution; Porphyromonas gingivalis; Reactive oxygen species

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