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Bio Protoc. 2017 Dec 20;7(24):e2648. doi: 10.21769/BioProtoc.2648. eCollection 2017 Dec 20.

Immunogold Electron Microscopy of the Autophagosome Marker LC3.

Bio-protocol

Domenico Mattoscio, Andrea Raimondi, Carlo Tacchetti, Susanna Chiocca

Affiliations

  1. Department of Medical, Oral, and Biotechnology Science, University of Chieti-Pescara, Chieti, Italy.
  2. Center on Aging Science and Translational Medicine (CeSI-MeT) "G. d'Annunzio", University of Chieti-Pescara, Chieti, Italy.
  3. Experimental Imaging Center, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  4. Medical School, University Vita.Salute San Raffaele, Milan, Italy.
  5. Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.

PMID: 34595311 PMCID: PMC8438370 DOI: 10.21769/BioProtoc.2648

Abstract

Even though autophagy was firstly observed by transmission electron microscopy already in the 1950s (reviewed in Eskelinen

Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Keywords: Autophagosome; Autophagy; Immunogold electron microscopy; LC3; Viral carcinogenesis

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