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van Suylen V, Vandendriessche K, Neyrinck A, et al. Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts. Artif Organs. 2021;doi: 10.1111/aor.14085.
van Suylen, V., Vandendriessche, K., Neyrinck, A., Nijhuis, F., van der Plaats, A., Verbeken, E. K., Vermeersch, P., Meyns, B., Mariani, M. A., Rega, F., & Erasmus, M. E. (2021). Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts. Artificial organs, . https://doi.org/10.1111/aor.14085
van Suylen, Vincent, et al. "Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts." Artificial organs vol. (2021). doi: https://doi.org/10.1111/aor.14085
van Suylen V, Vandendriessche K, Neyrinck A, Nijhuis F, van der Plaats A, Verbeken EK, Vermeersch P, Meyns B, Mariani MA, Rega F, Erasmus ME. Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts. Artif Organs. 2021 Oct 11; doi: 10.1111/aor.14085. Epub 2021 Oct 11. PMID: 34633676.
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Artif Organs. 2021 Oct 11; doi: 10.1111/aor.14085. Epub 2021 Oct 11.

Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts.

Artificial organs

Vincent van Suylen, Katrien Vandendriessche, Arne Neyrinck, Foppe Nijhuis, Arjan van der Plaats, Erik K Verbeken, Pieter Vermeersch, Bart Meyns, Massimo A Mariani, Filip Rega, Michiel E Erasmus

Affiliations

  1. Department of Cardiothoracic Surgery, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
  2. Department of Cardiovascular Sciences, Catholic University Leuven, Leuven, Belgium.
  3. Laboratory of Experimental Thoracic Surgery, Department of Clinical and Experimental Medicine, Catholic University Leuven, Leuven, Belgium.
  4. Department of Anesthesiology, University Hospitals Leuven, Leuven, Belgium.
  5. Organ Assist, Groningen, the Netherlands.
  6. Department of Histopathology, University Hospitals Leuven, Leuven, Belgium.
  7. Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium.
  8. Department of Cardiac Surgery, University Hospitals Leuven, Leuven, Belgium.

PMID: 34633676 DOI: 10.1111/aor.14085

Abstract

BACKGROUND: There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.

METHODS: Cardioplegia-arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.

RESULTS: For both groups, in vivo baseline and post-Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (p = 0.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30 min up to 90 min of reperfusion. Contractility was superior in MP during the first hour of evaluation.

CONCLUSION: We conclude that the initial functional outcome of MP-preserved hearts was transiently superior compared to CS, with no histological injury post-Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization.

© 2021 The Authors. Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.

Keywords: evaluation; ex situ heart perfusion; machine perfusion; preservation; temperature

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