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Swinnen G, Mauxion JP, Baekelandt A, et al. SlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato. Plant Physiol. 2021;doi: 10.1093/plphys/kiab464.
Swinnen, G., Mauxion, J. P., Baekelandt, A., De Clercq, R., Van Doorsselaere, J., Inzé, D., Gonzalez, N., Goossens, A., & Pauwels, L. (2021). SlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato. Plant physiology, . https://doi.org/10.1093/plphys/kiab464
Swinnen, Gwen, et al. "SlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato." Plant physiology vol. (2021). doi: https://doi.org/10.1093/plphys/kiab464
Swinnen G, Mauxion JP, Baekelandt A, De Clercq R, Van Doorsselaere J, Inzé D, Gonzalez N, Goossens A, Pauwels L. SlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato. Plant Physiol. 2021 Oct 02; doi: 10.1093/plphys/kiab464. Epub 2021 Oct 02. PMID: 34601614.
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Plant Physiol. 2021 Oct 02; doi: 10.1093/plphys/kiab464. Epub 2021 Oct 02.

SlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato.

Plant physiology

Gwen Swinnen, Jean-Philippe Mauxion, Alexandra Baekelandt, Rebecca De Clercq, Jan Van Doorsselaere, Dirk Inzé, Nathalie Gonzalez, Alain Goossens, Laurens Pauwels

Affiliations

  1. Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.
  2. VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  3. INRAE, Université de Bordeaux, BFP, 33882 Villenave d'Ornon Cedex, France.
  4. VIVES, 8800 Roeselare, Belgium.

PMID: 34601614 DOI: 10.1093/plphys/kiab464

Abstract

Plant organ size and shape are major agronomic traits that depend on cell division and expansion, which are both regulated by complex gene networks. In several eudicot species belonging to the rosid clade, organ growth is controlled by a repressor complex consisting of PEAPOD (PPD) and KINASE-INDUCIBLE DOMAIN INTERACTING (KIX) proteins. The role of these proteins in asterids, which together with the rosids constitute most of the core eudicot species, is unknown. We used CRISPR-Cas9 genome editing to target SlKIX8 and SlKIX9 in the asterid model species tomato (Solanum lycopersicum) and analyzed loss-of-function phenotypes. Loss of function of SlKIX8 and SlKIX9 led to the production of enlarged, dome-shaped leaves and these leaves exhibited increased expression of putative SlPPD target genes. Unexpectedly, kix8 kix9 mutants carried enlarged fruits with increased pericarp thickness due to cell expansion. At the molecular level, protein interaction assays indicated that SlKIX8 and SlKIX9 act as adaptors between the SlPPD and SlTOPLESS co-repressor proteins. Our results show that KIX8 and KIX9 are regulators of organ growth in asterids and can be used in strategies to improve important traits in produce such as thickness of the fruit flesh.

© The Author(s) (2021). Published by Oxford University Press on behalf of American Society of Plant Biologists.

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