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Animals (Basel). 2021 Aug 24;11(9). doi: 10.3390/ani11092477.

Determining the Effects of Serial Injections of Pregnant Mare Serum Gonadotropin on Plasma Testosterone Concentrations, Testicular Dynamics, and Semen Production in Leopard Geckos (.

Animals : an open access journal from MDPI

Alexandra K Mason, Jeongha Lee, Sean M Perry, Kimberly L Boykin, Fabio Del Piero, Michael Lierz, Mark A Mitchell

Affiliations

  1. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
  2. Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
  3. Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
  4. Mississippi Aquarium, Gulfport, MS 39501, USA.
  5. Clinic for Birds, Reptiles, Amphibians, and Fish, Justus Liebig University Giessen, 35392 Giessen, Germany.

PMID: 34573443 PMCID: PMC8470321 DOI: 10.3390/ani11092477

Abstract

Reptiles are highly susceptible to anthropogenic activities as a result of their narrow geographical ranges and habitat specialization, making them a conservation concern. Geckos represent one of the mega-diverse reptile lineages under pressure; however, limited assisted reproductive technologies currently exist for these animals. Exogenous pregnant mare serum gonadotropin (PMSG) has been found to exhibit follicle stimulating hormone-like action and has been routinely used to alter reproductive hormones of vertebrates in assisted reproductive protocols. The purpose of this study was to determine the effects of serial injections of 20 IU and 50 IU PMSG on circulating testosterone concentrations, testicular dynamics, and semen production in a model species of gecko. Twenty-four captive-bred, adult, male leopard geckos (

Keywords: Eublepharis macularius; PMSG; leopard gecko; pregnant mare serum gonadotropin; semen; testicle; testosterone

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