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Henning H, Luther AM, Waberski D. A High Incidence of Sperm with Cytoplasmic Droplets Affects the Response to Bicarbonate in Preserved Boar Semen. Animals (Basel). 2021;11(9)doi: 10.3390/ani11092570.
Henning, H., Luther, A. M., & Waberski, D. (2021). A High Incidence of Sperm with Cytoplasmic Droplets Affects the Response to Bicarbonate in Preserved Boar Semen. Animals : an open access journal from MDPI, 11(9), . https://doi.org/10.3390/ani11092570
Henning, Heiko, et al. "A High Incidence of Sperm with Cytoplasmic Droplets Affects the Response to Bicarbonate in Preserved Boar Semen." Animals : an open access journal from MDPI vol. 11,9 (2021). doi: https://doi.org/10.3390/ani11092570
Henning H, Luther AM, Waberski D. A High Incidence of Sperm with Cytoplasmic Droplets Affects the Response to Bicarbonate in Preserved Boar Semen. Animals (Basel). 2021 Aug 31;11(9). doi: 10.3390/ani11092570. PMID: 34573536; PMCID: PMC8465936.
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Animals (Basel). 2021 Aug 31;11(9). doi: 10.3390/ani11092570.

A High Incidence of Sperm with Cytoplasmic Droplets Affects the Response to Bicarbonate in Preserved Boar Semen.

Animals : an open access journal from MDPI

Heiko Henning, Anne-Marie Luther, Dagmar Waberski

Affiliations

  1. Unit for Reproductive Medicine, Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany.
  2. Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, 31535 Neustadt am Rübenberge, Germany.

PMID: 34573536 PMCID: PMC8465936 DOI: 10.3390/ani11092570

Abstract

Retained cytoplasmic droplets (CD) are the most frequent sperm abnormality in boar semen. A high incidence of CD is associated with subfertility, but the underlaying reasons are not well understood. The storage of extended semen might augment the adverse effects of CD on essential steps towards fertilization, such as capacitation. The aim of this study was to examine whether the enhanced presence of CD in boar semen influences sperm's response to the capacitation stimulus bicarbonate during long-term semen storage. Extended semen samples (n = 78) from 13 artificial insemination centers were analyzed using a flow cytometric calcium influx assay. Samples with >15% of CD showed a reduced specific response to bicarbonate and a higher non-specific destabilization after storage for 96 h and subsequent incubation at 38 °C in three variants of Tyrode's medium (

Keywords: boar semen; calcium influx; capacitation; cytoplasmic droplets; semen preservation

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