Display options
Cite
Burlage LC, Lellouch AG, Taveau CB, et al. Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts. J Surg Res. 2021;270:151-161doi: 10.1016/j.jss.2021.09.005.
Burlage, L. C., Lellouch, A. G., Taveau, C. B., Tratnig-Frankl, P., Pendexter, C. A., Randolph, M. A., Porte, R. J., Lantieri, L. A., Tessier, S. N., Cetrulo, C. L., & Uygun, K. (2021). Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts. The Journal of surgical research, 270151-161. https://doi.org/10.1016/j.jss.2021.09.005
Burlage, Laura C, et al. "Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts." The Journal of surgical research vol. 270 (2021): 151-161. doi: https://doi.org/10.1016/j.jss.2021.09.005
Burlage LC, Lellouch AG, Taveau CB, Tratnig-Frankl P, Pendexter CA, Randolph MA, Porte RJ, Lantieri LA, Tessier SN, Cetrulo CL, Uygun K. Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts. J Surg Res. 2021 Oct 17;270:151-161. doi: 10.1016/j.jss.2021.09.005. Epub 2021 Oct 17. PMID: 34670191.
Copy Download .nbib Format: NLM
Share it on

J Surg Res. 2021 Oct 17;270:151-161. doi: 10.1016/j.jss.2021.09.005. Epub 2021 Oct 17.

Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts.

The Journal of surgical research

Laura C Burlage, Alexandre G Lellouch, Corentin B Taveau, Philipp Tratnig-Frankl, Casie A Pendexter, Mark A Randolph, Robert J Porte, Laurent A Lantieri, Shannon N Tessier, Curtis L Cetrulo, Korkut Uygun

Affiliations

  1. Center for Engineering in Medicine and Surgery, Department of Surgery, Massachusetts General Hospital/ Harvard Medical School, Boston, Massachusetts; Department of Surgery, University Medical Center Groningen, Groningen, Netherlands; Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, Massachusetts; Division of Plastic and Reconstructive Surgery within the Department of Surgery, Massachusetts General Hospital/ Harvard Medical School, Boston, Massachusetts; Shriners Hospitals for Children, Boston, Massachusetts. Electronic address: [email protected].
  2. Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, Massachusetts; Division of Plastic and Reconstructive Surgery within the Department of Surgery, Massachusetts General Hospital/ Harvard Medical School, Boston, Massachusetts; Shriners Hospitals for Children, Boston, Massachusetts; Division of Plastic and Reconstructive Surgery within the Department of Surgery, European George Pompidou Hospital, University of Paris, Paris, France.
  3. Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, Massachusetts; Division of Plastic and Reconstructive Surgery within the Department of Surgery, Massachusetts General Hospital/ Harvard Medical School, Boston, Massachusetts; Shriners Hospitals for Children, Boston, Massachusetts.
  4. Center for Engineering in Medicine and Surgery, Department of Surgery, Massachusetts General Hospital/ Harvard Medical School, Boston, Massachusetts; Shriners Hospitals for Children, Boston, Massachusetts.
  5. Department of Surgery, University Medical Center Groningen, Groningen, Netherlands.
  6. Division of Plastic and Reconstructive Surgery within the Department of Surgery, European George Pompidou Hospital, University of Paris, Paris, France.

PMID: 34670191 DOI: 10.1016/j.jss.2021.09.005

Abstract

BACKGROUND: Machine perfusion is gaining interest as an efficient method of tissue preservation of Vascularized Composite Allografts (VCA). The aim of this study was to develop a protocol for ex vivo subnormothermic oxygenated machine perfusion (SNMP) on rodent hindlimbs and to validate our protocol in a heterotopic hindlimb transplant model.

METHODS: In this optimization study we compared three different solutions during 6 h of SNMP (n = 4 per group). Ten control limbs were stored in a preservation solution on Static Cold Storage [SCS]). During SNMP we monitored arterial flowrate, lactate levels, and edema. After SNMP, muscle biopsies were taken for histology examination, and energy charge analysis. We validated the best perfusion protocol in a heterotopic limb transplantation model with 30-d follow up (n = 13). As controls, we transplanted untreated limbs (n = 5) and hindlimbs preserved with either 6 or 24 h of SCS (n = 4 and n = 5).

RESULTS: During SNMP, arterial outflow increased, and lactate clearance decreased in all groups. Total edema was significantly lower in the HBOC-201 group compared to the BSA group (P = 0.005), 4.9 (4.3-6.1) versus 48.8 (39.1-53.2) percentage, but not to the BSA + PEG group (P = 0.19). Energy charge levels of SCS controls decreased 4-fold compared to limbs perfused with acellular oxygen carrier HBOC-201, 0.10 (0.07-0.17) versus 0.46 (0.42-0.49) respectively (P = 0.002).

CONCLUSIONS: Six hours ex vivo SNMP of rodent hindlimbs using an acellular oxygen carrier HBOC-201 results in superior tissue preservation compared to conventional SCS.

Copyright © 2021. Published by Elsevier Inc.

Keywords: energy charge; face transplantation; free flap; limb transplantation; oxygen carrier; oxygenated machine perfusion; plastic surgery; preservation; vascularized composite allotransplantation

Publication Types

Grant support