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Morohoshi K, Yamazaki T, Kito K, et al. Identification of an antibacterial polypeptide in mouse seminal vesicle secretions. J Reprod Immunol. 2021;148:103436doi: 10.1016/j.jri.2021.103436.
Morohoshi, K., Yamazaki, T., Kito, K., Sato, B., Kang, W., Hibino, T., Yoshida, M., Yoshida, K., Iwamoto, T., Yamada, M., Miyado, K., & Kawano, N. (2021). Identification of an antibacterial polypeptide in mouse seminal vesicle secretions. Journal of reproductive immunology, 148103436. https://doi.org/10.1016/j.jri.2021.103436
Morohoshi, Kazunori, et al. "Identification of an antibacterial polypeptide in mouse seminal vesicle secretions." Journal of reproductive immunology vol. 148 (2021): 103436. doi: https://doi.org/10.1016/j.jri.2021.103436
Morohoshi K, Yamazaki T, Kito K, Sato B, Kang W, Hibino T, Yoshida M, Yoshida K, Iwamoto T, Yamada M, Miyado K, Kawano N. Identification of an antibacterial polypeptide in mouse seminal vesicle secretions. J Reprod Immunol. 2021 Nov;148:103436. doi: 10.1016/j.jri.2021.103436. Epub 2021 Oct 19. PMID: 34700103.
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J Reprod Immunol. 2021 Nov;148:103436. doi: 10.1016/j.jri.2021.103436. Epub 2021 Oct 19.

Identification of an antibacterial polypeptide in mouse seminal vesicle secretions.

Journal of reproductive immunology

Kazunori Morohoshi, Takeo Yamazaki, Keiji Kito, Ban Sato, Woojin Kang, Taku Hibino, Manabu Yoshida, Kaoru Yoshida, Teruaki Iwamoto, Mitsutoshi Yamada, Kenji Miyado, Natsuko Kawano

Affiliations

  1. Laboratory of Regulatory Biology, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa, 214-8571, Japan.
  2. Laboratory of Proteomics, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa, 214-8571, Japan.
  3. Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan.
  4. Faculty of Education, Saitama University, 255 Shimo-Okubo, Sakura, Saitama City, Saitama, 338-8570, Japan.
  5. Misaki Marine Biological Station, School of Science, the University of Tokyo, 1024 Koajiro, Misaki, Miura, Kanagawa, 238-0225, Japan.
  6. Faculty of Biomedical Engineering, Toin University of Yokohama, 1614 Kurogane, Aoba, Yokohama, Kanagawa, 225-8503, Japan.
  7. Division of Male Infertility, Center for Human Reproduction, Sanno Hospital, International University of Health and Welfare, 8-10-21 Akasaka, Minato, Tokyo, 107-0052, Japan.
  8. Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, 160-8582, Japan.
  9. Laboratory of Regulatory Biology, Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa, 214-8571, Japan. Electronic address: [email protected].

PMID: 34700103 DOI: 10.1016/j.jri.2021.103436

Abstract

In both men and women, pathogenic bacteria enter the reproductive tract and cause harmful symptoms. Intrauterine and oviductal inflammation after copulation may have severe effects, such as infertility, implantation failure, oviduct obstruction, and robust life-threatening bacterial infection. Human seminal plasma is considered to be protective against bacterial infection. Among its components, Semenogelin-I/-II proteins are digested to function as bactericidal factors; however, their sequences are not conserved in mammals. Therefore, alternative antibacterial (bactericidal and/or bacteriostatic) systems may exist across mammals. In this study, we examined the antibacterial activity in the seminal plasma of mice lacking a gene cluster encoding Semenogelin-I/-II counterparts. Even in the absence of the majority of seminal proteins, antibacterial activity remained in the seminal plasma. Moreover, a combination of gel chromatography and liquid chromatography coupled with tandem mass spectrometry revealed that the prostate and testis expressed 4 protein as a novel antibacterial (specifically, bacteriostatic) protein, the sequence of which is broadly conserved across mammals. Our results provide the first evidence of a bacteriostatic protein that is widely present in the mammalian seminal plasma.

Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Keywords: Antibacterial activity; Bacteriostasis; PATE4; SVS7; Seminal plasma; Seminal vesicle secretion

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