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Xiong R, Hua D, Van Hoeck J, et al. Photothermal nanofibres enable safe engineering of therapeutic cells. Nat Nanotechnol. 2021;16(11):1281-1291doi: 10.1038/s41565-021-00976-3.
Xiong, R., Hua, D., Van Hoeck, J., Berdecka, D., Léger, L., De Munter, S., Fraire, J. C., Raes, L., Harizaj, A., Sauvage, F., Goetgeluk, G., Pille, M., Aalders, J., Belza, J., Van Acker, T., Bolea-Fernandez, E., Si, T., Vanhaecke, F., De Vos, W. H., Vandekerckhove, B., van Hengel, J., Raemdonck, K., Huang, C., De Smedt, S. C., & Braeckmans, K. (2021). Photothermal nanofibres enable safe engineering of therapeutic cells. Nature nanotechnology, 16(11), 1281-1291. https://doi.org/10.1038/s41565-021-00976-3
Xiong, Ranhua, et al. "Photothermal nanofibres enable safe engineering of therapeutic cells." Nature nanotechnology vol. 16,11 (2021): 1281-1291. doi: https://doi.org/10.1038/s41565-021-00976-3
Xiong R, Hua D, Van Hoeck J, Berdecka D, Léger L, De Munter S, Fraire JC, Raes L, Harizaj A, Sauvage F, Goetgeluk G, Pille M, Aalders J, Belza J, Van Acker T, Bolea-Fernandez E, Si T, Vanhaecke F, De Vos WH, Vandekerckhove B, van Hengel J, Raemdonck K, Huang C, De Smedt SC, Braeckmans K. Photothermal nanofibres enable safe engineering of therapeutic cells. Nat Nanotechnol. 2021 Nov;16(11):1281-1291. doi: 10.1038/s41565-021-00976-3. Epub 2021 Oct 21. PMID: 34675410; PMCID: PMC7612007.
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Nat Nanotechnol. 2021 Nov;16(11):1281-1291. doi: 10.1038/s41565-021-00976-3. Epub 2021 Oct 21.

Photothermal nanofibres enable safe engineering of therapeutic cells.

Nature nanotechnology

Ranhua Xiong, Dawei Hua, Jelter Van Hoeck, Dominika Berdecka, Laurens Léger, Stijn De Munter, Juan C Fraire, Laurens Raes, Aranit Harizaj, Félix Sauvage, Glenn Goetgeluk, Melissa Pille, Jeffrey Aalders, Joke Belza, Thibaut Van Acker, Eduardo Bolea-Fernandez, Ting Si, Frank Vanhaecke, Winnok H De Vos, Bart Vandekerckhove, Jolanda van Hengel, Koen Raemdonck, Chaobo Huang, Stefaan C De Smedt, Kevin Braeckmans

Affiliations

  1. Joint Laboratory of Advanced Biomedical Materials (Nanjing Forestry University-Ghent University), International Innovation for Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing, People's Republic of China. [email protected].
  2. Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium. [email protected].
  3. Joint Laboratory of Advanced Biomedical Materials (Nanjing Forestry University-Ghent University), International Innovation for Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing, People's Republic of China.
  4. Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  5. Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
  6. Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.
  7. Department of Diagnostic Sciences and Cancer Research Institute Ghent, Ghent University, Ghent, Belgium.
  8. Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.
  9. Department of Chemistry, Atomic and Mass Spectrometry Research Group, Ghent University, Ghent, Belgium.
  10. Department of Modern Mechanics, University of Science and Technology of China, Hefei, People's Republic of China.
  11. Joint Laboratory of Advanced Biomedical Materials (Nanjing Forestry University-Ghent University), International Innovation for Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing, People's Republic of China. [email protected].
  12. Joint Laboratory of Advanced Biomedical Materials (Nanjing Forestry University-Ghent University), International Innovation for Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing, People's Republic of China. [email protected].
  13. Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium. [email protected].
  14. Center for Advanced Light Microscopy, Ghent University, Ghent, Belgium. [email protected].
  15. Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium. [email protected].
  16. Center for Advanced Light Microscopy, Ghent University, Ghent, Belgium. [email protected].

PMID: 34675410 PMCID: PMC7612007 DOI: 10.1038/s41565-021-00976-3

Abstract

Nanoparticle-sensitized photoporation is an upcoming approach for the intracellular delivery of biologics, combining high efficiency and throughput with excellent cell viability. However, as it relies on close contact between nanoparticles and cells, its translation towards clinical applications is hampered by safety and regulatory concerns. Here we show that light-sensitive iron oxide nanoparticles embedded in biocompatible electrospun nanofibres induce membrane permeabilization by photothermal effects without direct cellular contact with the nanoparticles. The photothermal nanofibres have been successfully used to deliver effector molecules, including CRISPR-Cas9 ribonucleoprotein complexes and short interfering RNA, to adherent and suspension cells, including embryonic stem cells and hard-to-transfect T cells, without affecting cell proliferation or phenotype. In vivo experiments furthermore demonstrated successful tumour regression in mice treated with chimeric antibody receptor T cells in which the expression of programmed cell death protein 1 (PD1) is downregulated after nanofibre photoporation with short interfering RNA to PD1. In conclusion, cell membrane permeabilization with photothermal nanofibres is a promising concept towards the safe and more efficient production of engineered cells for therapeutic applications, including stem cell or adoptive T cell therapy.

© 2021. The Author(s), under exclusive licence to Springer Nature Limited.

References

  1. ACS Nano. 2012 Jul 24;6(7):5767-83 - PubMed
  2. Am J Med. 2004 Mar 15;116(6):394-401 - PubMed
  3. Opt Express. 2012 Oct 8;20(21):22976-86 - PubMed
  4. Stem Cells Dev. 2008 Aug;17(4):837-48 - PubMed
  5. Int J Mol Sci. 2020 Jan 30;21(3): - PubMed
  6. Nat Rev Immunol. 2009 Oct;9(10):704-16 - PubMed
  7. Nat Rev Genet. 2014 Aug;15(8):541-55 - PubMed
  8. Nat Med. 2015 Feb;21(2):121-31 - PubMed
  9. Pharmacol Ther. 2020 May;209:107501 - PubMed
  10. Adv Drug Deliv Rev. 2014 May;71:98-114 - PubMed
  11. Nat Protoc. 2007;2(4):792-6 - PubMed
  12. Biomaterials. 2012 Mar;33(7):2345-50 - PubMed
  13. Nanotechnology. 2014 Jun 20;25(24):245101 - PubMed
  14. ACS Nano. 2014 Mar 25;8(3):2889-99 - PubMed
  15. Nat Nanotechnol. 2010 Aug;5(8):607-11 - PubMed
  16. Nano Lett. 2016 Oct 12;16(10):5975-5986 - PubMed
  17. Immunol Rev. 2013 May;253(1):185-97 - PubMed
  18. Int J Mol Sci. 2018 Jan 30;19(2): - PubMed
  19. Adv Mater. 2012 Jul 24;24(28):3831-7 - PubMed
  20. J Immunol. 2002 Nov 15;169(10):5754-60 - PubMed
  21. Anal Biochem. 1983 Oct 1;134(1):111-6 - PubMed
  22. Chem Soc Rev. 2011 May;40(5):2417-34 - PubMed
  23. Proc Natl Acad Sci U S A. 2018 Nov 13;115(46):E10907-E10914 - PubMed
  24. J Immunol Methods. 2014 Jun;408:123-31 - PubMed
  25. Science. 2019 Mar 29;363(6434): - PubMed
  26. Methods Enzymol. 2012;509:195-224 - PubMed
  27. Nature. 2014 Feb 6;506(7486):52-7 - PubMed
  28. Biomater Sci. 2021 Jun 7;9(11):4005-4018 - PubMed
  29. Nature. 2016 Oct 12;538(7624):183-192 - PubMed
  30. Chem Soc Rev. 2013 Nov 7;42(21):8339-59 - PubMed
  31. Chem Res Toxicol. 1992 Mar-Apr;5(2):227-31 - PubMed
  32. Cell Stem Cell. 2020 Oct 1;27(4):523-531 - PubMed
  33. Nat Nanotechnol. 2021 Aug;16(8):926-932 - PubMed
  34. PLoS One. 2016 May 19;11(5):e0154806 - PubMed
  35. Nat Commun. 2019 Apr 9;10(1):1634 - PubMed
  36. Stem Cell Rev Rep. 2012 Sep;8(3):768-78 - PubMed
  37. Nat Biotechnol. 2019 Aug;37(8):895-906 - PubMed
  38. ACS Nano. 2014 Jun 24;8(6):6288-96 - PubMed
  39. Chem Rev. 2018 Aug 22;118(16):7409-7531 - PubMed
  40. Light Sci Appl. 2018 Aug 8;7:47 - PubMed

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