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Naya-Català F, do Vale Pereira G, Piazzon MC, et al. Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (. Front Physiol. 2021;12:748265doi: 10.3389/fphys.2021.748265.
Naya-Català, F., do Vale Pereira, G., Piazzon, M. C., Fernandes, A. M., Calduch-Giner, J. A., Sitjà-Bobadilla, A., Conceição, L. E. C., & Pérez-Sánchez, J. (2021). Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (. Frontiers in physiology, 12748265. https://doi.org/10.3389/fphys.2021.748265
Naya-Català, Fernando, et al. "Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (." Frontiers in physiology vol. 12 (2021): 748265. doi: https://doi.org/10.3389/fphys.2021.748265
Naya-Català F, do Vale Pereira G, Piazzon MC, Fernandes AM, Calduch-Giner JA, Sitjà-Bobadilla A, Conceição LEC, Pérez-Sánchez J. Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (. Front Physiol. 2021 Oct 05;12:748265. doi: 10.3389/fphys.2021.748265. eCollection 2021. PMID: 34675821; PMCID: PMC8523787.
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Front Physiol. 2021 Oct 05;12:748265. doi: 10.3389/fphys.2021.748265. eCollection 2021.

Cross-Talk Between Intestinal Microbiota and Host Gene Expression in Gilthead Sea Bream (.

Frontiers in physiology

Fernando Naya-Català, Gabriella do Vale Pereira, M Carla Piazzon, Ana Margarida Fernandes, Josep Alvar Calduch-Giner, Ariadna Sitjà-Bobadilla, Luis E C Conceição, Jaume Pérez-Sánchez

Affiliations

  1. Nutrigenomics and Fish Growth Endocrinology Group, Institute of Aquaculture Torre de la Sal (IATS-CSIC), Castellón, Spain.
  2. SPAROS Lda, Area Empresarial de Marim, Olh?o, Portugal.
  3. Fish Pathology Group, Institute of Aquaculture Torre de la Sal (IATS-CSIC), Castellón, Spain.
  4. Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.

PMID: 34675821 PMCID: PMC8523787 DOI: 10.3389/fphys.2021.748265

Abstract

New types of fish feed based on processed animal proteins (PAPs), insect meal, yeast, and microbial biomasses have been used with success in gilthead sea bream. However, some drawback effects on feed conversion and inflammatory systemic markers were reported in different degrees with PAP- and non-PAP-based feed formulations. Here, we focused on the effects of control and two experimental diets on gut mucosal-adherent microbiota, and how it correlated with host transcriptomics at the local (intestine) and systemic (liver and head kidney) levels. The use of tissue-specific PCR-arrays of 93 genes in total rendered 13, 12, and 9 differentially expressed (DE) genes in the intestine, liver, and head kidney, respectively. Illumina sequencing of gut microbiota yielded a mean of 125,350 reads per sample, assigned to 1,281 operational taxonomic unit (OTUs). Bacterial richness and alpha diversity were lower in fish fed with the PAP diet, and discriminant analysis displayed 135 OTUs driving the separation between groups with 43 taxa correlating with 27 DE genes. The highest expression of intestinal

Copyright © 2021 Naya-Català, do Vale Pereira, Piazzon, Fernandes, Calduch-Giner, Sitjà-Bobadilla, Conceição and Pérez-Sánchez.

Keywords: algae meal; fish meal; gut microbiota; host transcriptomics; inflammatory markers; insect proteins; lipid metabolism; processed animal proteins

Conflict of interest statement

GV, AF, and LC were employed by the company SPAROS (Olhăo, Portugal). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that cou

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