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Ronchi G, Tos P, Angelino E, et al. Effect of unacylated ghrelin on peripheral nerve regeneration. Eur J Histochem. 2021;65doi: 10.4081/ejh.2021.3287.
Ronchi, G., Tos, P., Angelino, E., Muratori, L., Reano, S., Filigheddu, N., Graziani, A., Geuna, S., & Raimondo, S. (2021). Effect of unacylated ghrelin on peripheral nerve regeneration. European journal of histochemistry : EJH, 65. https://doi.org/10.4081/ejh.2021.3287
Ronchi, Giulia, et al. "Effect of unacylated ghrelin on peripheral nerve regeneration." European journal of histochemistry : EJH vol. 65 (2021). doi: https://doi.org/10.4081/ejh.2021.3287
Ronchi G, Tos P, Angelino E, Muratori L, Reano S, Filigheddu N, Graziani A, Geuna S, Raimondo S. Effect of unacylated ghrelin on peripheral nerve regeneration. Eur J Histochem. 2021 Nov 04;65. doi: 10.4081/ejh.2021.3287. PMID: 34734521; PMCID: PMC8586818.
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Eur J Histochem. 2021 Nov 04;65. doi: 10.4081/ejh.2021.3287.

Effect of unacylated ghrelin on peripheral nerve regeneration.

European journal of histochemistry : EJH

Giulia Ronchi, Pierluigi Tos, Elia Angelino, Luisa Muratori, Simone Reano, Nicoletta Filigheddu, Andrea Graziani, Stefano Geuna, Stefania Raimondo

Affiliations

  1. Department of Clinical and Biological Sciences and Neuroscience Institute of the "Cavalieri Ottolenghi" Foundation (NICO), University of Turin, Orbassano (TO). [email protected].
  2. Hand Surgery and Reconstructive Microsurgery Unit, ASST G. Pini-CTO, Milan. [email protected].
  3. Department of Biotechnologies and Health Sciences, Molecular Biotechnology Center, University of Turin. [email protected].
  4. Department of Clinical and Biological Sciences and Neuroscience Institute of the "Cavalieri Ottolenghi" Foundation (NICO), University of Turin, Orbassano (TO). [email protected].
  5. Department of Translational Medicine, University of Piemonte Orientale, Novara. [email protected].
  6. Department of Translational Medicine, University of Piemonte Orientale, Novara. [email protected].
  7. Department of Biotechnologies and Health Sciences, Molecular Biotechnology Center, University of Turin. [email protected].
  8. Department of Clinical and Biological Sciences and Neuroscience Institute of the "Cavalieri Ottolenghi" Foundation (NICO), University of Turin, Orbassano (TO). [email protected].
  9. Department of Clinical and Biological Sciences and Neuroscience Institute of the "Cavalieri Ottolenghi" Foundation (NICO), University of Turin, Orbassano (TO). [email protected].

PMID: 34734521 PMCID: PMC8586818 DOI: 10.4081/ejh.2021.3287

Abstract

Ghrelin is a circulating peptide hormone released by enteroendocrine cells of the gastrointestinal tract as two forms, acylated and unacylated. Acylated ghrelin (AG) binds to the growth hormone secretagogue receptor 1a (GHSR1a), thus stimulating food intake, growth hormone release, and gastrointestinal motility. Conversely, unacylated GHR (UnAG), through binding to a yet unidentified receptor, protects the skeletal muscle from atrophy, stimulates muscle regeneration, and protects cardiomyocytes from ischemic damage. Recently, interest about ghrelin has raised also among neuroscientists because of its effect on the nervous system, especially the stimulation of neurogenesis in spinal cord, brain stem, and hippocampus. However, few information is still available about its effectiveness on peripheral nerve regeneration. To partially fill this gap, the aim of this study was to assess the effect of UnAG on peripheral nerve regeneration after median nerve crush injury and after nerve transection immediately repaired by means of an end-to-end suture. To this end, we exploited FVB1 Myh6/Ghrl transgenic mice in which overexpression of the ghrelin gene (Ghrl) results in selective up-regulation of circulating UnAG levels, but not of AG. Regeneration was assessed by both functional evaluation (grasping test) and morphometrical analysis of regenerated myelinated axons. Results obtained lead to conclude that UnAG could have a role in development of peripheral nerves and during more severe lesions.

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