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Bengtsen MB, Hansen ESS, Tougaard RS, et al. Hyperpolarized [1-. Exp Physiol. 2021;106(12):2412-2422doi: 10.1113/EP089782.
Bengtsen, M. B., Hansen, E. S. S., Tougaard, R. S., Lyhne, M. D., Rittig, N. F., Støy, J., Jessen, N., Mariager, C. �. �., Stødkilde-Jørgensen, H., Møller, N., & Laustsen, C. (2021). Hyperpolarized [1-. Experimental physiology, 106(12), 2412-2422. https://doi.org/10.1113/EP089782
Bengtsen, Mads Bisgaard, et al. "Hyperpolarized [1-." Experimental physiology vol. 106,12 (2021): 2412-2422. doi: https://doi.org/10.1113/EP089782
Bengtsen MB, Hansen ESS, Tougaard RS, Lyhne MD, Rittig NF, Støy J, Jessen N, Mariager CØ, Stødkilde-Jørgensen H, Møller N, Laustsen C. Hyperpolarized [1-. Exp Physiol. 2021 Dec;106(12):2412-2422. doi: 10.1113/EP089782. Epub 2021 Nov 15. PMID: 34705304.
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Exp Physiol. 2021 Dec;106(12):2412-2422. doi: 10.1113/EP089782. Epub 2021 Nov 15.

Hyperpolarized [1-.

Experimental physiology

Mads Bisgaard Bengtsen, Esben Søvsø Szocska Hansen, Rasmus Stilling Tougaard, Mads Dam Lyhne, Nikolaj Fibiger Rittig, Julie Støy, Niels Jessen, Christian Østergaard Mariager, Hans Stødkilde-Jørgensen, Niels Møller, Christoffer Laustsen

Affiliations

  1. Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus N, Denmark.
  2. The MR Research Center, Aarhus University Hospital, Aarhus N, Denmark.
  3. Department of Cardiology, Aarhus University Hospital, Aarhus N, Denmark.
  4. Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus N, Denmark.

PMID: 34705304 DOI: 10.1113/EP089782

Abstract

NEW FINDINGS: What is the central question of this study? Is it possible to combine the hyperpolarized magnetic resonance technique and the hyperinsulinaemic clamp method in order to evaluate skeletal muscle metabolism in a large animal model? What is the main finding and its importance? The logistical set-up is possible, and we found substantial increments in glucose infusion rates representing skeletal muscle glucose uptake but no differences in ratios of [1-

ABSTRACT: In skeletal muscle, glucose metabolism is tightly regulated by the reciprocal relationship between insulin and adrenaline, with pyruvate being at the intersection of both pathways. Hyperpolarized magnetic resonance (hMR) is a new approach to gain insights into these pathways, and human trials involving hMR and skeletal muscle metabolism are imminent. We aimed to combine the hyperinsulinaemic clamp technique and hMR in a large animal model resembling human physiology. Fifteen anaesthetized pigs were randomized to saline (control group), hyperinsulinaemic euglycaemic clamp technique (HE group) or hyperinsulinaemic hypoglycaemic clamp technique (HH group). Skeletal muscle metabolism was evaluated by hyperpolarized [1-

© 2021 The Authors. Experimental Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.

Keywords: glucose metabolism; homeostasis; insulin; skeletal muscle

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