Display options
Cite
Tarbit E, Singh I, Peart JN, et al. Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts. Sci Rep. 2021;11(1):20376doi: 10.1038/s41598-021-99632-y.
Tarbit, E., Singh, I., Peart, J. N., Bivol, S., & Rose'Meyer, R. B. (2021). Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts. Scientific reports, 11(1), 20376. https://doi.org/10.1038/s41598-021-99632-y
Tarbit, Emiri, et al. "Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts." Scientific reports vol. 11,1 (2021): 20376. doi: https://doi.org/10.1038/s41598-021-99632-y
Tarbit E, Singh I, Peart JN, Bivol S, Rose'Meyer RB. Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts. Sci Rep. 2021 Oct 13;11(1):20376. doi: 10.1038/s41598-021-99632-y. PMID: 34645867; PMCID: PMC8514503.
Copy Download .nbib Format: NLM
Share it on

Sci Rep. 2021 Oct 13;11(1):20376. doi: 10.1038/s41598-021-99632-y.

Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts.

Scientific reports

Emiri Tarbit, Indu Singh, Jason Nigel Peart, Svetlana Bivol, Roselyn Barbara Rose'Meyer

Affiliations

  1. School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, QLD, 4222, Australia.
  2. School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, QLD, 4222, Australia. [email protected].

PMID: 34645867 PMCID: PMC8514503 DOI: 10.1038/s41598-021-99632-y

Abstract

Elevated blood serotonin levels have been observed in patients with heart failure and serotonin has a role in pathological cardiac function. The serotonin receptor system was examined in adult rat isolated cardiac fibroblast and myofibroblast cells. This is one of the first studies that has investigated serotonin receptors and other proteins involved in the serotonin receptor system in rat cardiac fibroblast and myofibroblast cells. Rat primary cardiac fibroblasts were isolated and transformed into myofibroblasts using 5 ng/ml TGF-β1. Transformation of cells to myofibroblasts was confirmed with the presence of α-smooth muscle actin using Western blot. Serotonin metabolism and receptor protein expression was assessed using Western blot techniques and serotonin levels measured using ELISA. The 5-HT

© 2021. The Author(s).

References

  1. J Mol Cell Cardiol. 2009 Apr;46(4):518-25 - PubMed
  2. Arch Cardiovasc Dis. 2017 Jan;110(1):51-59 - PubMed
  3. J Cardiovasc Pharmacol. 2011 Apr;57(4):376-9 - PubMed
  4. Biomed Res Int. 2015;2015:438403 - PubMed
  5. Circulation. 2004 Aug 24;110(8):969-74 - PubMed
  6. Sci Rep. 2017 Nov 7;7(1):14761 - PubMed
  7. Pharmacol Ther. 2010 Dec;128(3):559-67 - PubMed
  8. J Heart Valve Dis. 2009 Jan;18(1):44-51 - PubMed
  9. Cardiovasc Res. 2005 Mar 1;65(4):869-78 - PubMed
  10. Circulation. 2001 Jun 19;103(24):2973-9 - PubMed
  11. Circulation. 2005 Mar 29;111(12):1517-22 - PubMed
  12. Circulation. 2021 Mar 30;143(13):1317-1330 - PubMed
  13. Circ Res. 2009 Dec 4;105(12):1164-76 - PubMed
  14. Circ Res. 2009 Jan 2;104(1):1-3 - PubMed
  15. Circ Res. 2009 Jan 2;104(1):113-23 - PubMed
  16. J Neural Transm (Vienna). 2013 Jun;120(6):927-35 - PubMed
  17. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9508-13 - PubMed
  18. Traffic. 2008 Sep;9(9):1393-402 - PubMed
  19. Biochim Biophys Acta. 2013 Apr;1833(4):945-53 - PubMed
  20. Am J Physiol Heart Circ Physiol. 2009 Aug;297(2):H821-8 - PubMed
  21. J Mol Cell Cardiol. 2009 Apr;46(4):587-95 - PubMed
  22. Biochim Biophys Acta. 2011 Jul;1813(7):1323-32 - PubMed
  23. Heart. 2004 Apr;90(4):464-70 - PubMed
  24. Heart Lung Circ. 2017 May;26(5):442-449 - PubMed

Publication Types