Display options
Cite
Alfaro-Mora Y, Domínguez-Gómez G, Cáceres-Gutiérrez RE, et al. MPS1 is involved in the HPV16-E7-mediated centrosomes amplification. Cell Div. 2021;16(1):6doi: 10.1186/s13008-021-00074-9.
Alfaro-Mora, Y., Domínguez-Gómez, G., Cáceres-Gutiérrez, R. E., Tolentino-García, L., Herrera, L. A., Castro-Hernández, C., Bermúdez-Cruz, R. M., & Díaz-Chávez, J. (2021). MPS1 is involved in the HPV16-E7-mediated centrosomes amplification. Cell division, 16(1), 6. https://doi.org/10.1186/s13008-021-00074-9
Alfaro-Mora, Yair, et al. "MPS1 is involved in the HPV16-E7-mediated centrosomes amplification." Cell division vol. 16,1 (2021): 6. doi: https://doi.org/10.1186/s13008-021-00074-9
Alfaro-Mora Y, Domínguez-Gómez G, Cáceres-Gutiérrez RE, Tolentino-García L, Herrera LA, Castro-Hernández C, Bermúdez-Cruz RM, Díaz-Chávez J. MPS1 is involved in the HPV16-E7-mediated centrosomes amplification. Cell Div. 2021 Nov 04;16(1):6. doi: 10.1186/s13008-021-00074-9. PMID: 34736484; PMCID: PMC8567613.
Copy Download .nbib Format: NLM
Share it on

Cell Div. 2021 Nov 04;16(1):6. doi: 10.1186/s13008-021-00074-9.

MPS1 is involved in the HPV16-E7-mediated centrosomes amplification.

Cell division

Yair Alfaro-Mora, Guadalupe Domínguez-Gómez, Rodrigo E Cáceres-Gutiérrez, Laura Tolentino-García, Luis A Herrera, Clementina Castro-Hernández, Rosa María Bermúdez-Cruz, José Díaz-Chávez

Affiliations

  1. Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Mexico City, Mexico.
  2. Unidad de Investigación Biomédica en Cáncer, Instituto de Investigaciones Biomédicas, UNAM/Instituto Nacional de Cancerología (INCan), Mexico City, Mexico.
  3. Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
  4. Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Mexico City, Mexico. [email protected].
  5. Unidad de Investigación Biomédica en Cáncer, Instituto de Investigaciones Biomédicas, UNAM/Instituto Nacional de Cancerología (INCan), Mexico City, Mexico. [email protected].

PMID: 34736484 PMCID: PMC8567613 DOI: 10.1186/s13008-021-00074-9

Abstract

BACKGROUND: It has been reported that the oncoprotein E7 from human papillomavirus type 16 (HPV16-E7) can induce the excessive synthesis of centrosomes through the increase in the expression of PLK4, which is a transcriptional target of E2F1. On the other hand, it has been reported that increasing MPS1 protein stability can also generate an excessive synthesis of centrosomes. In this work, we analyzed the possible role of MPS1 in the amplification of centrosomes mediated by HPV16-E7.

RESULTS: Employing qRT-PCR, Western Blot, and Immunofluorescence techniques, we found that E7 induces an increase in the MPS1 transcript and protein levels in the U2OS cell line, as well as protein stabilization. Besides, we observed that inhibiting the expression of MPS1 in E7 protein-expressing cells leads to a significant reduction in the number of centrosomes.

CONCLUSIONS: These results indicate that the presence of the MPS1 protein is necessary for E7 protein to increase the number of centrosomes, and possible implications are discussed.

© 2021. The Author(s).

Keywords: Centrosome; HPV16-E7; MPS1; PLK4

References

  1. Genes Cells. 2007 Jun;12(6):797-810 - PubMed
  2. Oncogene. 2014 Jul 31;33(31):4039-49 - PubMed
  3. Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10002-7 - PubMed
  4. Mol Biol Cell. 2010 Nov 15;21(22):3878-89 - PubMed
  5. Oncogene. 2002 Sep 9;21(40):6146-53 - PubMed
  6. Nat Rev Mol Cell Biol. 2015 Jan;16(1):5-17 - PubMed
  7. Cell Cycle. 2007 Aug 1;6(15):1903-13 - PubMed
  8. Mol Biol Cell. 2007 Nov;18(11):4457-69 - PubMed
  9. Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4 - PubMed
  10. J Cell Biol. 2002 Sep 30;158(7):1171-81 - PubMed
  11. J Cell Sci. 1996 Dec;109 ( Pt 13):3089-102 - PubMed
  12. Curr Biol. 1995 Dec 1;5(12):1384-93 - PubMed
  13. Oncogene. 1994 Jan;9(1):89-96 - PubMed
  14. Cancer Res. 2001 Mar 15;61(6):2356-60 - PubMed
  15. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2950-5 - PubMed
  16. J Cell Biol. 2013 Apr 15;201(2):217-31 - PubMed
  17. PLoS One. 2009 Aug 10;4(8):e6564 - PubMed
  18. Nature. 2009 Jul 9;460(7252):278-82 - PubMed
  19. Int J Cancer. 2005 Feb 20;113(5):746-51 - PubMed
  20. J Virol. 2003 Nov;77(22):12331-5 - PubMed
  21. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14875-80 - PubMed
  22. Mol Cell Proteomics. 2008 Sep;7(9):1598-608 - PubMed
  23. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7828-33 - PubMed
  24. Front Oncol. 2013 Nov 12;3:277 - PubMed
  25. Clin Cancer Res. 1999 May;5(5):963-70 - PubMed
  26. Chem Biol. 2011 Jan 28;18(1):111-20 - PubMed
  27. J Biol Chem. 2001 Oct 19;276(42):39012-20 - PubMed
  28. Nucleic Acids Res. 2016 Jan 8;44(1):164-74 - PubMed
  29. Cell. 2000 Sep 29;103(1):127-40 - PubMed
  30. Acta Virol. 2013;57(4):447-51 - PubMed
  31. Biochem J. 2009 Jan 1;417(1):173-81 - PubMed
  32. Cell. 2001 Jul 13;106(1):95-104 - PubMed
  33. Nucleic Acids Res. 2005 Jan 1;33(Database issue):D34-8 - PubMed
  34. Cell Rep. 2015 Jul 7;12(1):66-78 - PubMed
  35. J Natl Cancer Inst. 2013 Sep 4;105(17):1322-31 - PubMed
  36. Nucleic Acids Res. 2017 Jan 4;45(D1):D158-D169 - PubMed
  37. Nat Rev Mol Cell Biol. 2007 Jun;8(6):451-63 - PubMed
  38. Nucleic Acids Res. 2012 Feb;40(4):1561-78 - PubMed
  39. Cell Cycle. 2012 Oct 1;11(19):3666-78 - PubMed
  40. J Cell Biol. 2010 Jan 25;188(2):191-8 - PubMed
  41. Trends Cell Biol. 2008 Aug;18(8):389-96 - PubMed
  42. J Cell Biol. 2011 May 16;193(4):727-39 - PubMed
  43. Cell Mol Life Sci. 2013 Mar;70(6):1021-34 - PubMed
  44. Genes Dev. 2002 Jan 15;16(2):245-56 - PubMed
  45. Nat Cell Biol. 2012 Jun 03;14(7):746-52 - PubMed
  46. Biochem J. 2012 Dec 15;448(3):321-8 - PubMed
  47. Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E657-66 - PubMed
  48. Philos Trans R Soc Lond B Biol Sci. 2014 Sep 5;369(1650): - PubMed
  49. Oncogene. 2007 Jan 11;26(2):215-23 - PubMed
  50. Oncogene. 2006 May 11;25(20):2943-9 - PubMed
  51. Curr Biol. 2005 Dec 20;15(24):2199-207 - PubMed
  52. Nucleic Acids Res. 2014;42(16):10331-50 - PubMed
  53. Virology. 2009 Feb 20;384(2):335-44 - PubMed
  54. J Cell Biol. 2001 Apr 30;153(3):517-27 - PubMed
  55. Genes Dev. 2012 Mar 1;26(5):474-89 - PubMed
  56. J Virol. 1992 Dec;66(12):6893-902 - PubMed
  57. Methods. 2001 Dec;25(4):402-8 - PubMed
  58. EMBO J. 1994 Feb 15;13(4):871-8 - PubMed
  59. Oncogene. 2007 Sep 20;26(43):6280-8 - PubMed
  60. EMBO J. 2002 Apr 2;21(7):1723-32 - PubMed
  61. J Biol Chem. 2007 Oct 19;282(42):30553-61 - PubMed
  62. BMC Struct Biol. 2009 Jul 31;9:51 - PubMed
  63. J Virol. 2008 Sep;82(17):8695-705 - PubMed
  64. Oncogene. 2004 Oct 28;23(50):8206-15 - PubMed
  65. Cell Rep. 2015 Nov 24;13(8):1569-77 - PubMed
  66. Mol Cancer. 2011 May 24;10:61 - PubMed
  67. Dev Cell. 2002 Sep;3(3):339-50 - PubMed
  68. Cell Biol Int. 2005 May;29(5):375-83 - PubMed
  69. Mol Cell. 2007 May 25;26(4):539-51 - PubMed
  70. Nat Methods. 2012 Jul;9(7):671-5 - PubMed
  71. PLoS One. 2013 Jul 01;8(7):e67822 - PubMed
  72. J Biol Chem. 2010 Oct 22;285(43):32988-32998 - PubMed
  73. J Infect Dis. 2011 Nov 15;204(10):1505-13 - PubMed
  74. Curr Biol. 2010 Mar 23;20(6):R285-95 - PubMed
  75. Nucleic Acids Res. 2004 Mar 19;32(5):1792-7 - PubMed

Publication Types

Grant support