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Ruocco N, Esposito R, Zagami G, et al. Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis. Sci Rep. 2021;11(1):21151doi: 10.1038/s41598-021-00713-9.
Ruocco, N., Esposito, R., Zagami, G., Bertolino, M., De Matteo, S., Sonnessa, M., Andreani, F., Crispi, S., Zupo, V., & Costantini, M. (2021). Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis. Scientific reports, 11(1), 21151. https://doi.org/10.1038/s41598-021-00713-9
Ruocco, Nadia, et al. "Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis." Scientific reports vol. 11,1 (2021): 21151. doi: https://doi.org/10.1038/s41598-021-00713-9
Ruocco N, Esposito R, Zagami G, Bertolino M, De Matteo S, Sonnessa M, Andreani F, Crispi S, Zupo V, Costantini M. Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis. Sci Rep. 2021 Oct 27;11(1):21151. doi: 10.1038/s41598-021-00713-9. PMID: 34707182; PMCID: PMC8551288.
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Sci Rep. 2021 Oct 27;11(1):21151. doi: 10.1038/s41598-021-00713-9.

Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis.

Scientific reports

Nadia Ruocco, Roberta Esposito, Giacomo Zagami, Marco Bertolino, Sergio De Matteo, Michele Sonnessa, Federico Andreani, Stefania Crispi, Valerio Zupo, Maria Costantini

Affiliations

  1. Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Naples, Italy.
  2. Department of Biology, University of Naples Federico II, Complesso Universitario Di Monte Sant'Angelo, Via Cinthia 21, 80126, Naples, Italy.
  3. Dipartimento Di Scienze Biologiche, Chimiche, Farmaceutiche Ed Ambientali, Università Di Messina, 98100, Messina, Italy.
  4. DISTAV, Università Degli Studi Di Genova, Corso Europa 26, 16132, Genoa, Italy.
  5. Bio-Fab Research Srl, Via Mario Beltrami, 5, 00135, Rome, Italy.
  6. Institute of Biosciences and BioResources Naples, National Research Council of Italy, Naples, Italy.
  7. Department of Marine Biotechnology, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Naples, Italy. [email protected].

PMID: 34707182 PMCID: PMC8551288 DOI: 10.1038/s41598-021-00713-9

Abstract

Although the Mediterranean Sea covers approximately a 0.7% of the world's ocean area, it represents a major reservoir of marine and coastal biodiversity. Among marine organisms, sponges (Porifera) are a key component of the deep-sea benthos, widely recognized as the dominant taxon in terms of species richness, spatial coverage, and biomass. Sponges are evolutionarily ancient, sessile filter-feeders that harbor a largely diverse microbial community within their internal mesohyl matrix. In the present work, we firstly aimed at exploring the biodiversity of marine sponges from four different areas of the Mediterranean: Faro Lake in Sicily and "Porto Paone", "Secca delle fumose", "Punta San Pancrazio" in the Gulf of Naples. Eight sponge species were collected from these sites and identified by morphological analysis and amplification of several conserved molecular markers (18S and 28S RNA ribosomal genes, mitochondrial cytochrome oxidase subunit 1 and internal transcribed spacer). In order to analyze the bacterial diversity of symbiotic communities among these different sampling sites, we also performed a metataxonomic analysis through an Illumina MiSeq platform, identifying more than 1500 bacterial taxa. Amplicon Sequence Variants (ASVs) analysis revealed a great variability of the host-specific microbial communities. Our data highlight the occurrence of dominant and locally enriched microbes in the Mediterranean, together with the biotechnological potential of these sponges and their associated bacteria as sources of bioactive natural compounds.

© 2021. The Author(s).

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