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Lownik JC, Farrar JS, Way GW, et al. Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting. Diagnostics (Basel). 2021;11(10)doi: 10.3390/diagnostics11101788.
Lownik, J. C., Farrar, J. S., Way, G. W., McKay, A., Roychoudhury, P., Greninger, A. L., & Martin, R. K. (2021). Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting. Diagnostics (Basel, Switzerland), 11(10), . https://doi.org/10.3390/diagnostics11101788
Lownik, Joseph C, et al. "Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting." Diagnostics (Basel, Switzerland) vol. 11,10 (2021). doi: https://doi.org/10.3390/diagnostics11101788
Lownik JC, Farrar JS, Way GW, McKay A, Roychoudhury P, Greninger AL, Martin RK. Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting. Diagnostics (Basel). 2021 Sep 28;11(10). doi: 10.3390/diagnostics11101788. PMID: 34679489; PMCID: PMC8534650.
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Diagnostics (Basel). 2021 Sep 28;11(10). doi: 10.3390/diagnostics11101788.

Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting.

Diagnostics (Basel, Switzerland)

Joseph C Lownik, Jared S Farrar, Grayson W Way, Angela McKay, Pavitra Roychoudhury, Alexander L Greninger, Rebecca K Martin

Affiliations

  1. Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  2. Center for Clinical and Translation Research, Virginia Commonwealth University, Richmond, VA 23298, USA.
  3. Department of Laboratory Medicine and Pathology, University of Washington Medical Center, Seattle, WA 98102, USA.
  4. Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298, USA.

PMID: 34679489 PMCID: PMC8534650 DOI: 10.3390/diagnostics11101788

Abstract

SARS-CoV-2, the virus responsible for COVID-19, emerged in late 2019 and has since spread throughout the world, infecting over 200 million people. The fast spread of SARS-CoV-2 showcased the need for rapid and sensitive testing methodologies to help track the disease. Over the past 18 months, numerous SARS-CoV-2 variants have emerged. Many of these variants are suggested to be more transmissible as well as less responsive to neutralization by vaccine-induced antibodies. Viral whole-genome sequencing is the current standard for tracking these variants. However, whole-genome sequencing is costly and the technology and expertise are limited to larger reference laboratories. Here, we present the feasibility of a fast, inexpensive methodology using snapback primer-based high-resolution melting to test for >20 high-consequence SARS-CoV-2 spike mutations. This assay can distinguish between multiple variant lineages and be completed in roughly 2 h for less than $10 per sample.

Keywords: COVID testing; COVID-19; PCR; SARS-CoV-2; high-resolution melting; snapback primer

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