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Cuddihy J, Wu G, Ho L, et al. Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin. Sci Rep. 2021;11(1):21249doi: 10.1038/s41598-021-00644-5.
Cuddihy, J., Wu, G., Ho, L., Kudo, H., Dannhorn, A., Mandalia, S., Collins, D., Weir, J., Spencer, A., Vizcaychipi, M., Takats, Z., & Nagy, I. (2021). Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin. Scientific reports, 11(1), 21249. https://doi.org/10.1038/s41598-021-00644-5
Cuddihy, Joshua, et al. "Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin." Scientific reports vol. 11,1 (2021): 21249. doi: https://doi.org/10.1038/s41598-021-00644-5
Cuddihy J, Wu G, Ho L, Kudo H, Dannhorn A, Mandalia S, Collins D, Weir J, Spencer A, Vizcaychipi M, Takats Z, Nagy I. Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin. Sci Rep. 2021 Oct 28;11(1):21249. doi: 10.1038/s41598-021-00644-5. PMID: 34711882; PMCID: PMC8553775.
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Sci Rep. 2021 Oct 28;11(1):21249. doi: 10.1038/s41598-021-00644-5.

Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin.

Scientific reports

Joshua Cuddihy, Gongjie Wu, Laptin Ho, Hiromi Kudo, Andreas Dannhorn, Sundhiya Mandalia, Declan Collins, Justin Weir, Ashley Spencer, Marcela Vizcaychipi, Zoltan Takats, Istvan Nagy

Affiliations

  1. Department of Surgery and Cancer, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, Exhibition Road, London, SW7 2BU, UK. [email protected].
  2. Magill Department of Anaesthetics, Critical Care and Pain Medicine, Chelsea and Westminster Hospital NHS Foundation Trust, 369 Fulham Road, London, SW10 9NH, UK. [email protected].
  3. Department of Surgery and Cancer, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, Exhibition Road, London, SW7 2BU, UK.
  4. Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2BU, UK.
  5. Research and Development, Chelsea and Westminster Hospital NHS Foundation Trust, 369 Fulham Road, London, SW10 9NH, UK.
  6. Department of Burns Surgery, Chelsea and Westminster Hospital NHS Foundation Trust, 369 Fulham Road, London, SW10 9NH, UK.
  7. Department of Dermatology, Imperial College Healthcare NHS Trust, Du Cane Road, London, W12 0HS, UK.
  8. Department of Cellular Pathology, North West London Pathology, Charing Cross Hospital, London, W8 8RF, UK.
  9. Magill Department of Anaesthetics, Critical Care and Pain Medicine, Chelsea and Westminster Hospital NHS Foundation Trust, 369 Fulham Road, London, SW10 9NH, UK.

PMID: 34711882 PMCID: PMC8553775 DOI: 10.1038/s41598-021-00644-5

Abstract

Burn injuries constitute one of the most serious accidental injuries. Increased metabolic rate is a hallmark feature of burn injury. Visualising lactate dehydrogenase (LDH) activity has been previously used to identify metabolic activity differences, hence cell viability and burn depth in burn skin. LDH activity was visualised in injured and uninjured skin from 38 sub-acute burn patients. LDH activity aided the identification of spatially correlating immunocompetent cells in a sub-group of six patients. Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI MSI) was used to describe relative lactate and pyruvate abundance in burned and uninjured tissue. LDH activity was significantly increased in the middle and deep regions of burnt skin compared with superficial areas in burnt skin and uninjured tissue and positively correlated with post-burn time. Regions of increased LDH activity showed high pyruvate and low lactate abundance when examined with DESI-MSI. Areas of increased LDH activity exhibited cellular infiltration, including CD3 + and CD4 + T-lymphocytes and CD68 + macrophages. Our data demonstrate a steady increase in functional LDH activity in sub-acute burn wounds linked to cellular infiltration. The cell types associated are related to tissue restructuring and inflammation. This region in burn wounds is likely the focus of dysregulated inflammation and hypermetabolism.

© 2021. The Author(s).

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