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Lee E, Kim YS, Lee YM, et al. Identification of stemness and differentially expressed genes in human cementum-derived cells. J Periodontal Implant Sci. 2021;51(5):329-341doi: 10.5051/jpis.2102600130.
Lee, E., Kim, Y. S., Lee, Y. M., Kim, W. K., Lee, Y. K., & Kim, S. H. (2021). Identification of stemness and differentially expressed genes in human cementum-derived cells. Journal of periodontal & implant science, 51(5), 329-341. https://doi.org/10.5051/jpis.2102600130
Lee, EunHye, et al. "Identification of stemness and differentially expressed genes in human cementum-derived cells." Journal of periodontal & implant science vol. 51,5 (2021): 329-341. doi: https://doi.org/10.5051/jpis.2102600130
Lee E, Kim YS, Lee YM, Kim WK, Lee YK, Kim SH. Identification of stemness and differentially expressed genes in human cementum-derived cells. J Periodontal Implant Sci. 2021 Oct;51(5):329-341. doi: 10.5051/jpis.2102600130. PMID: 34713994; PMCID: PMC8558007.
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J Periodontal Implant Sci. 2021 Oct;51(5):329-341. doi: 10.5051/jpis.2102600130.

Identification of stemness and differentially expressed genes in human cementum-derived cells.

Journal of periodontal & implant science

EunHye Lee, Young-Sung Kim, Yong-Moo Lee, Won-Kyung Kim, Young-Kyoo Lee, Su-Hwan Kim

Affiliations

  1. Dental Research Institute, Seoul National University, Seoul, Korea.
  2. Department of Periodontics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
  3. Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.
  4. Department of Dentistry, Uijeongbu Eulji Medical Center, Eulji University, Uijeongbu, Korea.
  5. Department of Periodontics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. [email protected].

PMID: 34713994 PMCID: PMC8558007 DOI: 10.5051/jpis.2102600130

Abstract

PURPOSE: Periodontal treatment aims at complete regeneration of the periodontium, and developing strategies for periodontal regeneration requires a deep understanding of the tissues composing the periodontium. In the present study, the stemness characteristics and gene expression profiles of cementum-derived cells (CDCs) were investigated and compared with previously established human stem cells. Candidate marker proteins for CDCs were also explored.

METHODS: Periodontal ligament stem cells (PDLSCs), pulp stem cells (PULPSCs), and CDCs were isolated and cultured from extracted human mandibular third molars. Human bone marrow stem cells (BMSCs) were used as a positive control. To identify the stemness of CDCs, cell differentiation (osteogenic, adipogenic, and chondrogenic) and surface antigens were evaluated through flow cytometry. The expression of cementum protein 1 (CEMP1) and cementum attachment protein (CAP) was investigated to explore marker proteins for CDCs through reverse-transcription polymerase chain reaction. To compare the gene expression profiles of the 4 cell types, mRNA and miRNA microarray analysis of 10 samples of BMSCs (n=1), PDLSCs (n=3), PULPSCs (n=3), and CDCs (n=3) were performed.

RESULTS: The expression of mesenchymal stem cell markers with a concomitant absence of hematopoietic markers was observed in PDLSCs, PULPSCs, CDCs and BMSCs. All 4 cell populations also showed differentiation into osteogenic, adipogenic, and chondrogenic lineages. CEMP1 was strongly expressed in CDCs, while it was weakly detected in the other 3 cell populations. Meanwhile, CAP was not found in any of the 4 cell populations. The mRNA and miRNA microarray analysis showed that 14 mRNA genes and 4 miRNA genes were differentially expressed in CDCs vs. PDLSCs and PULPSCs.

CONCLUSIONS: Within the limitations of the study, CDCs seem to have stemness and preferentially express CEMP1. Moreover, there were several up- or down-regulated genes in CDCs vs. PDLSCs, PULPSCs, and BMSCs and these genes could be candidate marker proteins of CDCs.

Copyright © 2021. Korean Academy of Periodontology.

Keywords: Dental cementum; MicroRNAs; Microarray analysis; RNA, messenger; Stem cells

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

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