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Berrío RT, Verstaen K, Vandamme N, et al. Single-Cell Transcriptomics Sheds Light on the Identity and Metabolism of Developing Leaf Cells. Plant Physiol. 2021;doi: 10.1093/plphys/kiab489.
Berrío, R. T., Verstaen, K., Vandamme, N., Pevernagie, J., Achon, I., Van Duyse, J., Van Isterdael, G., Saeys, Y., De Veylder, L., Inzé, D., & Dubois, M. (2021). Single-Cell Transcriptomics Sheds Light on the Identity and Metabolism of Developing Leaf Cells. Plant physiology, . https://doi.org/10.1093/plphys/kiab489
Berrío, Rubén Tenorio, et al. "Single-Cell Transcriptomics Sheds Light on the Identity and Metabolism of Developing Leaf Cells." Plant physiology vol. (2021). doi: https://doi.org/10.1093/plphys/kiab489
Berrío RT, Verstaen K, Vandamme N, Pevernagie J, Achon I, Van Duyse J, Van Isterdael G, Saeys Y, De Veylder L, Inzé D, Dubois M. Single-Cell Transcriptomics Sheds Light on the Identity and Metabolism of Developing Leaf Cells. Plant Physiol. 2021 Oct 23; doi: 10.1093/plphys/kiab489. Epub 2021 Oct 23. PMID: 34687312.
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Plant Physiol. 2021 Oct 23; doi: 10.1093/plphys/kiab489. Epub 2021 Oct 23.

Single-Cell Transcriptomics Sheds Light on the Identity and Metabolism of Developing Leaf Cells.

Plant physiology

Rubén Tenorio Berrío, Kevin Verstaen, Niels Vandamme, Julie Pevernagie, Ignacio Achon, Julie Van Duyse, Gert Van Isterdael, Yvan Saeys, Lieven De Veylder, Dirk Inzé, Marieke Dubois

Affiliations

  1. Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
  2. VIB Center for Plant Systems Biology, Ghent, Belgium.
  3. Ghent University, Department of Applied Mathematics, Computer Science and Statistics, Ghent, Belgium.
  4. VIB Center for Inflammation Research, Ghent, Belgium.
  5. Ghent University, Department of Biomedical Molecular Biology, Ghent, Belgium.

PMID: 34687312 DOI: 10.1093/plphys/kiab489

Abstract

As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization of leaf mesophyll and epidermal cell layers orchestrates photosynthesis and gas exchange. In addition, water and nutrients for leaf growth are transported through the vascular tissue. To establish the single-cell transcriptomic landscape of these different leaf tissues, we performed high-throughput transcriptome sequencing of individual cells isolated from young leaves of Arabidopsis (Arabidopsis thaliana) seedlings grown in two different environmental conditions. The detection of ∼19,000 different transcripts in over 1,800 high-quality leaf cells revealed 14 cell populations composing the young, differentiating leaf. Besides the cell populations comprising the core leaf tissues, we identified subpopulations with distinct identity or metabolic activity. In addition, we proposed cell-type specific markers for each of these populations. Finally, an intuitive web tool allows for browsing the presented dataset. Our data presents insights on how the different cell populations constituting a developing leaf are connected via developmental, metabolic, or stress-related trajectories.

© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please email: [email protected].

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