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Warner RM, Shuttleworth R, Benson JD, et al. General tissue mass transfer model for cryopreservation applications. Biophys J. 2021;120(22):4980-4991doi: 10.1016/j.bpj.2021.10.014.
Warner, R. M., Shuttleworth, R., Benson, J. D., Eroglu, A., & Higgins, A. Z. (2021). General tissue mass transfer model for cryopreservation applications. Biophysical journal, 120(22), 4980-4991. https://doi.org/10.1016/j.bpj.2021.10.014
Warner, Ross M, et al. "General tissue mass transfer model for cryopreservation applications." Biophysical journal vol. 120,22 (2021): 4980-4991. doi: https://doi.org/10.1016/j.bpj.2021.10.014
Warner RM, Shuttleworth R, Benson JD, Eroglu A, Higgins AZ. General tissue mass transfer model for cryopreservation applications. Biophys J. 2021 Nov 16;120(22):4980-4991. doi: 10.1016/j.bpj.2021.10.014. Epub 2021 Oct 16. PMID: 34662558; PMCID: PMC8633834.
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Biophys J. 2021 Nov 16;120(22):4980-4991. doi: 10.1016/j.bpj.2021.10.014. Epub 2021 Oct 16.

General tissue mass transfer model for cryopreservation applications.

Biophysical journal

Ross M Warner, Robyn Shuttleworth, James D Benson, Ali Eroglu, Adam Z Higgins

Affiliations

  1. School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon.
  2. Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
  3. Department of Neuroscience and Regenerative Medicine, Medical College of Georgia - Augusta University, Augusta, Georgia.
  4. School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon. Electronic address: [email protected].

PMID: 34662558 PMCID: PMC8633834 DOI: 10.1016/j.bpj.2021.10.014

Abstract

Successful cryopreservation of complex specimens, such as tissues and organs, would greatly benefit both the medical and scientific research fields. Vitrification is one of the most promising techniques for complex specimen cryopreservation, but toxicity remains a major challenge because of the high concentration of cryoprotectants (CPAs) needed to vitrify. Our group has approached this problem using mathematical optimization to design less toxic CPA equilibration methods for cells. To extend this approach to tissues, an appropriate mass transfer model is required. Fick's law is commonly used, but this simple modeling framework does not account for the complexity of mass transfer in tissues, such as the effects of fixed charges, tissue size changes, and the interplay between cell membrane transport and transport through the extracellular fluid. Here, we propose a general model for mass transfer in tissues that accounts for all of these phenomena. To create this model, we augmented a previously published acellular model of mass transfer in articular cartilage to account for the effects of cells. We show that the model can accurately predict changes in CPA concentration and tissue size for both articular cartilage and pancreatic islets, tissue types with vastly different properties.

Copyright © 2021 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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