Display options
Cite
Zhou J, Alvarado-Kristensson M. Optimization of production of recombinant gamma-tubulin in bacteria. MethodsX. 2021;8:101517doi: 10.1016/j.mex.2021.101517.
Zhou, J., & Alvarado-Kristensson, M. (2021). Optimization of production of recombinant gamma-tubulin in bacteria. MethodsX, 8101517. https://doi.org/10.1016/j.mex.2021.101517
Zhou, Jingkai, and Alvarado-Kristensson, Maria. "Optimization of production of recombinant gamma-tubulin in bacteria." MethodsX vol. 8 (2021): 101517. doi: https://doi.org/10.1016/j.mex.2021.101517
Zhou J, Alvarado-Kristensson M. Optimization of production of recombinant gamma-tubulin in bacteria. MethodsX. 2021 Sep 16;8:101517. doi: 10.1016/j.mex.2021.101517. eCollection 2021. PMID: 34754788; PMCID: PMC8563660.
Copy Download .nbib Format: NLM
Share it on

MethodsX. 2021 Sep 16;8:101517. doi: 10.1016/j.mex.2021.101517. eCollection 2021.

Optimization of production of recombinant gamma-tubulin in bacteria.

MethodsX

Jingkai Zhou, Maria Alvarado-Kristensson

Affiliations

  1. Molecular Pathology, Department of Translational Medicine, Lund University, Jan Waldenströms gata 59, Malmö SE-205 02, Sweden.

PMID: 34754788 PMCID: PMC8563660 DOI: 10.1016/j.mex.2021.101517

Abstract

Production of a protein of interest in bacteria and its purification from bacterial lysates are valuable tools for the purification of larger amounts of recombinant proteins. The low cost of culturing, and the rapid cell growth of bacteria make this host a good choice for protein production, but the folding and function of the purified protein might be altered due to the production of a eukaryotic protein in a prokaryotic host. Here, we provide a purification method for the purification of gamma (γ)-tubulin (TUBG) from soluble fractions of

© 2021 The Authors. Published by Elsevier B.V.

Keywords: Bacteria; Gamma-tubulin; Native protein; Protein purification; Recombinant protein

References

  1. J Cell Biol. 1993 Sep;122(6):1301-10 - PubMed
  2. J Cell Sci. 1996 Apr;109 ( Pt 4):875-87 - PubMed
  3. Commun Biol. 2021 Jun 22;4(1):767 - PubMed
  4. Heliyon. 2016 Sep 24;2(9):e00166 - PubMed
  5. Nat Cell Biol. 2009 Sep;11(9):1081-92 - PubMed
  6. Cell. 1994 Feb 25;76(4):623-37 - PubMed
  7. Nature. 1995 Dec 7;378(6557):578-83 - PubMed
  8. Oncogene. 2005 Aug 4;24(33):5165-72 - PubMed
  9. Signal Transduct Target Ther. 2018 Sep 14;3:24 - PubMed
  10. Nature. 2010 Aug 12;466(7308):879-82 - PubMed
  11. FASEB J. 2011 Nov;25(11):3815-27 - PubMed
  12. Exp Cell Res. 2000 Apr 10;256(1):122-30 - PubMed
  13. Int J Mol Sci. 2018 Oct 19;19(10): - PubMed
  14. FASEB J. 2002 Jan;16(1):129-31 - PubMed
  15. Biochim Biophys Acta Proteins Proteom. 2018 Apr;1866(4):519-526 - PubMed
  16. Commun Biol. 2018 May 3;1:37 - PubMed

Publication Types