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Gong P, Bontinck M, Demuynck K, et al. SAMBA controls cell division rate during maize development. Plant Physiol. 2021;doi: 10.1093/plphys/kiab514.
Gong, P., Bontinck, M., Demuynck, K., De Block, J., Gevaert, K., Eeckhout, D., Persiau, G., Aesaert, S., Coussens, G., Van Lijsebettens, M., Pauwels, L., De Jaeger, G., Inzé, D., & Nelissen, H. (2021). SAMBA controls cell division rate during maize development. Plant physiology, . https://doi.org/10.1093/plphys/kiab514
Gong, Pan, et al. "SAMBA controls cell division rate during maize development." Plant physiology vol. (2021). doi: https://doi.org/10.1093/plphys/kiab514
Gong P, Bontinck M, Demuynck K, De Block J, Gevaert K, Eeckhout D, Persiau G, Aesaert S, Coussens G, Van Lijsebettens M, Pauwels L, De Jaeger G, Inzé D, Nelissen H. SAMBA controls cell division rate during maize development. Plant Physiol. 2021 Nov 13; doi: 10.1093/plphys/kiab514. Epub 2021 Nov 13. PMID: 34791456.
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Plant Physiol. 2021 Nov 13; doi: 10.1093/plphys/kiab514. Epub 2021 Nov 13.

SAMBA controls cell division rate during maize development.

Plant physiology

Pan Gong, Michiel Bontinck, Kirin Demuynck, Jolien De Block, Kris Gevaert, Dominique Eeckhout, Geert Persiau, Stijn Aesaert, Griet Coussens, Mieke Van Lijsebettens, Laurens Pauwels, Geert De Jaeger, Dirk Inzé, Hilde Nelissen

Affiliations

  1. Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.
  2. VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
  3. Department of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.
  4. VIB Center for Medical Biotechnology, 9000 Ghent, Belgium.

PMID: 34791456 DOI: 10.1093/plphys/kiab514

Abstract

SAMBA has been identified as a plant-specific regulator of the anaphase-promoting complex/cyclosome (APC/C) that controls unidirectional cell cycle progression in Arabidopsis (Arabidopsis thaliana), but so far its role has not been studied in monocots. Here, we show the association of SAMBA with the APC/C is conserved in maize (Zea mays). Two samba CRISPR mutants showed growth defects, such as reduced internode length, shortened upper leaves with erect leaf architecture, and reduced leaf size due to an altered cell division rate and cell expansion, which aggravated with plant age. The two mutants differed in the severity and developmental onset of the phenotypes, because samba-1 represented a knock-out allele, while translation re-initiation in samba-3 resulted in a truncated protein that was still able to interact with the APC/C and regulate its function, albeit with altered APC/C activity and efficiency. Our data are consistent with a dosage-dependent role for SAMBA to control developmental processes for which a change in growth rate is pivotal.

© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please email: [email protected].

Keywords: anaphase-promoting complex; cell division rate; genome editing; maize development; translation re-initiation

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