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Jayasena Kaluarachchi TD, Campbell PM, Wickremasinghe R, et al. Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds. Sci Rep. 2021;11(1):23181doi: 10.1038/s41598-021-02388-8.
Jayasena Kaluarachchi, T. D., Campbell, P. M., Wickremasinghe, R., Ranasinghe, S., Wickremasinghe, R., Yasawardene, S., De Silva, H., Menike, C., Jayarathne, M. C. K., Jayathilake, S., Dilhari, A., McBain, A. J., & Weerasekera, M. M. (2021). Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds. Scientific reports, 11(1), 23181. https://doi.org/10.1038/s41598-021-02388-8
Jayasena Kaluarachchi, T D, et al. "Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds." Scientific reports vol. 11,1 (2021): 23181. doi: https://doi.org/10.1038/s41598-021-02388-8
Jayasena Kaluarachchi TD, Campbell PM, Wickremasinghe R, Ranasinghe S, Wickremasinghe R, Yasawardene S, De Silva H, Menike C, Jayarathne MCK, Jayathilake S, Dilhari A, McBain AJ, Weerasekera MM. Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds. Sci Rep. 2021 Nov 30;11(1):23181. doi: 10.1038/s41598-021-02388-8. PMID: 34848752; PMCID: PMC8633208.
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Sci Rep. 2021 Nov 30;11(1):23181. doi: 10.1038/s41598-021-02388-8.

Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds.

Scientific reports

T D Jayasena Kaluarachchi, Paul M Campbell, Rajitha Wickremasinghe, Shalindra Ranasinghe, Renu Wickremasinghe, Surangi Yasawardene, Hiromel De Silva, Chandrani Menike, M C K Jayarathne, Subodha Jayathilake, Ayomi Dilhari, Andrew J McBain, Manjula M Weerasekera

Affiliations

  1. Department of Parasitology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka. [email protected].
  2. Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.
  3. Department of Public Health, Faculty of Medicine, University of Kelaniya, Kelaniya, Sri Lanka.
  4. Department of Parasitology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  5. Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  6. Dermatology Unit, Base Hospital, Tangalle, Sri Lanka.
  7. Department of Family Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  8. Department of Pathology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  9. Department of Basic Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  10. Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.

PMID: 34848752 PMCID: PMC8633208 DOI: 10.1038/s41598-021-02388-8

Abstract

The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin.

© 2021. The Author(s).

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