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Di Marzo M, Viana VE, Banfi C, et al. Cell wall modifications by α-XYLOSIDASE1 are required for the control of seed and fruit size. J Exp Bot. 2021;doi: 10.1093/jxb/erab514.
Di Marzo, M., Viana, V. E., Banfi, C., Cassina, V., Corti, R., Herrera-Ubaldo, H., Babolin, N., Guazzotti, A., Kiegle, E., Gregis, V., de Folter, S., Sampedro, J., Mantegazza, F., Colombo, L., & Ezquer, I. (2021). Cell wall modifications by α-XYLOSIDASE1 are required for the control of seed and fruit size. Journal of experimental botany, . https://doi.org/10.1093/jxb/erab514
Di Marzo, Maurizio, et al. "Cell wall modifications by α-XYLOSIDASE1 are required for the control of seed and fruit size." Journal of experimental botany vol. (2021). doi: https://doi.org/10.1093/jxb/erab514
Di Marzo M, Viana VE, Banfi C, Cassina V, Corti R, Herrera-Ubaldo H, Babolin N, Guazzotti A, Kiegle E, Gregis V, de Folter S, Sampedro J, Mantegazza F, Colombo L, Ezquer I. Cell wall modifications by α-XYLOSIDASE1 are required for the control of seed and fruit size. J Exp Bot. 2021 Nov 25; doi: 10.1093/jxb/erab514. Epub 2021 Nov 25. PMID: 34849721.
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J Exp Bot. 2021 Nov 25; doi: 10.1093/jxb/erab514. Epub 2021 Nov 25.

Cell wall modifications by α-XYLOSIDASE1 are required for the control of seed and fruit size.

Journal of experimental botany

Maurizio Di Marzo, Vívian Ebeling Viana, Camilla Banfi, Valeria Cassina, Roberta Corti, Humberto Herrera-Ubaldo, Nicola Babolin, Andrea Guazzotti, Edward Kiegle, Veronica Gregis, Stefan de Folter, Javier Sampedro, Francesco Mantegazza, Lucia Colombo, Ignacio Ezquer

Affiliations

  1. Dipartimento di BioScienze, Università degli Studi di Milano, Via Giovanni Celoria, Milan, Italy.
  2. Plant Genomics and Breeding Center, Federal University of Pelotas, Capão do Leão - RS, Brazil.
  3. School of Medicine and Surgery, Nanomedicine Center NANOMIB, University of Milan-Bicocca, Monza, Italy.
  4. Department of Materials Science, University of Milan-Bicocca, Milan, Italy.
  5. Unidad de Genómica Avanzada (UGA-LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, Guanajuato, México.
  6. Universidad de Santiago de Compostela, Departamento de Fisiología Vegetal, Facultad de Biología, Rúa Lope Gómez de Marzoa, s/n. Campus sur, Santiago de Compostela, A Coruña, Spain.

PMID: 34849721 DOI: 10.1093/jxb/erab514

Abstract

Cell wall modifications are of pivotal importance during plant development. Among cell wall components, xyloglucans are the major hemicellulose polysaccharide in primary cell walls of dicots and non graminaceous monocots. They can connect the cellulose microfibril surface to affect cell wall mechanical properties. Changes in xyloglucan structure are known to play an important role regulating cell growth. Therefore, the degradation of xyloglucan is an important modification that alters the cell wall. The α-XYLOSIDASE1 (XYL1) gene encodes the only α-xylosidase acting on xyloglucans in Arabidopsis thaliana. Here, we show that mutation of XYL1 strongly influences seed size, seed germination, and fruit elongation. We found that the expression of XYL1 is directly regulated in developing seeds and fruit by the MADS-box transcription factor SEEDSTICK (STK). We demonstrate that XYL1 complements the stk smaller seed phenotype. Finally, by Atomic Force Microscopy (AFM), we investigate the role of XYL1 activity in maintaining cell stiffness and growth, confirming the importance of cell wall modulation in shaping organs.

© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: [email protected].

Keywords: Cell wall; MADS-box; fruit growth; seed size; transcription factor; xyloglucan

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