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Landau Albrecht C, Sperling LE, Iglesias Braghirolli D, et al. Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine. Bioengineering (Basel). 2021;8(11)doi: 10.3390/bioengineering8110172.
Landau Albrecht, C., Sperling, L. E., Iglesias Braghirolli, D., & Pranke, P. (2021). Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine. Bioengineering (Basel, Switzerland), 8(11), . https://doi.org/10.3390/bioengineering8110172
Landau Albrecht, Carolina, et al. "Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine." Bioengineering (Basel, Switzerland) vol. 8,11 (2021). doi: https://doi.org/10.3390/bioengineering8110172
Landau Albrecht C, Sperling LE, Iglesias Braghirolli D, Pranke P. Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine. Bioengineering (Basel). 2021 Nov 03;8(11). doi: 10.3390/bioengineering8110172. PMID: 34821738; PMCID: PMC8614771.
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Bioengineering (Basel). 2021 Nov 03;8(11). doi: 10.3390/bioengineering8110172.

Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine.

Bioengineering (Basel, Switzerland)

Carolina Landau Albrecht, Laura Elena Sperling, Daikelly Iglesias Braghirolli, Patricia Pranke

Affiliations

  1. Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90.610-000, RS, Brazil.
  2. Postgraduate Program in Physiology, UFRGS, Porto Alegre 90.610-000, RS, Brazil.
  3. Stem Cell Research Institute (Instituto de Pesquisa com Células-Tronco), Porto Alegre 90.610-000, RS, Brazil.

PMID: 34821738 PMCID: PMC8614771 DOI: 10.3390/bioengineering8110172

Abstract

(1) Background: Nanotechnology is an emerging field that can be applied in the biomedical area. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine is the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine may be used as an adjuvant therapy in the treatment of inflammation in the central nervous system. (2) Methods: Nanocapsules were prepared by the interfacial deposition of the pre-formed polymer method and characterized in terms of zeta potential, diameter, polydispersity index, pH, encapsulation efficiency (EE), stability and sustained release profile. In vitro tests with the PC12 cell line were performed, such as MTT, LIVE/DEAD and ELISA assays, to verify their cytotoxic and anti-inflammatory effects. (3) Results: The nanocapsules presented satisfactory values of the characterization parameters; however, poor encapsulation was obtained for nicotine (8.17% ± 0.47). The in vitro tests showed that the treatment with nanocapsules reduced cell viability, which suggests that the Eudragit or the amount of polymer on top of the cells may be detrimental to them, as the cells were able to survive when treated with bulk nicotine. ELISA showed an increment in the expression of IL-6 and IL-1β, corroborating the hypothesis that NCs were toxic to the cells because of the increase in the levels of these pro-inflammatory cytokines. (4) Conclusions: This study demonstrates that NCs of Eudragit present toxicity. It is therefore necessary to improve NC formulation to obtain better values for the encapsulation efficiency and reduce toxicity of these nanodevices.

Keywords: Eudragit; PC12; inflammation; nanocapsules; nicotine

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