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Darnaud M, De Vadder F, Bogeat P, et al. A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes. Nat Commun. 2021;12(1):6686doi: 10.1038/s41467-021-26963-9.
Darnaud, M., De Vadder, F., Bogeat, P., Boucinha, L., Bulteau, A. L., Bunescu, A., Couturier, C., Delgado, A., Dugua, H., Elie, C., Mathieu, A., Novotná, T., Ouattara, D. A., Planel, S., Saliou, A., Šrůtková, D., Yansouni, J., Stecher, B., Schwarzer, M., Leulier, F., & Tamellini, A. (2021). A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes. Nature communications, 12(1), 6686. https://doi.org/10.1038/s41467-021-26963-9
Darnaud, Marion, et al. "A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes." Nature communications vol. 12,1 (2021): 6686. doi: https://doi.org/10.1038/s41467-021-26963-9
Darnaud M, De Vadder F, Bogeat P, Boucinha L, Bulteau AL, Bunescu A, Couturier C, Delgado A, Dugua H, Elie C, Mathieu A, Novotná T, Ouattara DA, Planel S, Saliou A, Šrůtková D, Yansouni J, Stecher B, Schwarzer M, Leulier F, Tamellini A. A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes. Nat Commun. 2021 Nov 18;12(1):6686. doi: 10.1038/s41467-021-26963-9. PMID: 34795236; PMCID: PMC8602333.
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Nat Commun. 2021 Nov 18;12(1):6686. doi: 10.1038/s41467-021-26963-9.

A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes.

Nature communications

Marion Darnaud, Filipe De Vadder, Pascaline Bogeat, Lilia Boucinha, Anne-Laure Bulteau, Andrei Bunescu, Céline Couturier, Ana Delgado, Hélène Dugua, Céline Elie, Alban Mathieu, Tereza Novotná, Djomangan Adama Ouattara, Séverine Planel, Adrien Saliou, Dagmar Šrůtková, Jennifer Yansouni, Bärbel Stecher, Martin Schwarzer, François Leulier, Andrea Tamellini

Affiliations

  1. BIOASTER, Institut de Recherche Technologique, 40 avenue Tony Garnier, 69007, Lyon, France. [email protected].
  2. Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Unité Mixte de Recherche 5242, 46 Allée d'Italie, 69364, Lyon, Cedex, 07, France.
  3. BIOASTER, Institut de Recherche Technologique, 40 avenue Tony Garnier, 69007, Lyon, France.
  4. Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, 54922, Nový Hrádek, Czech Republic.
  5. Max von Pettenkofer Institute of Hygiene and Medical Microbiology, Ludwig-Maximilians-University of Munich, 80336, Munich, Germany.
  6. German Center for Infection Research (DZIF), Partner Site, Munich, Germany.

PMID: 34795236 PMCID: PMC8602333 DOI: 10.1038/s41467-021-26963-9

Abstract

Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut microbiome in preclinical mouse studies, here we report the development of a simplified mouse microbiota composed of 15 strains from 7 of the 20 most prevalent bacterial families representative of the fecal microbiota of C57BL/6J Specific (and Opportunistic) Pathogen-Free (SPF/SOPF) animals and the derivation of a standardized gnotobiotic mouse model called GM15. GM15 recapitulates extensively the functionalities found in the C57BL/6J SOPF microbiota metagenome, and GM15 animals are phenotypically similar to SOPF or SPF animals in two different facilities. They are also less sensitive to the deleterious effects of post-weaning malnutrition. In this work, we show that the GM15 model provides increased reproducibility and robustness of preclinical studies by limiting the confounding effect of fluctuation in microbiota composition, and offers opportunities for research focused on how the microbiota shapes host physiology in health and disease.

© 2021. The Author(s).

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