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Cold Spring Harb Protoc. 2021 Dec 01;2021(12):pdb.top099945. doi: 10.1101/pdb.top099945.

Selecting the Antigen.

Cold Spring Harbor protocols

Edward A Greenfield, James DeCaprio, Mohan Brahmandam

PMID: 34853124 DOI: 10.1101/pdb.top099945

Abstract

The classical method for generating polyclonal or monoclonal antibodies relies on the in vivo humoral response of animals. Here we describe the factors that antigens can have that might influence the strength and quality of an antibody response. This introduction is divided into three sections: (1) an overview of immunogenicity, (2) choosing the best form for the immunogen, and (3) methods for modifying antigens to make them more immunogenic.

© 2021 Cold Spring Harbor Laboratory Press.

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