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Il'in DA, Shkurupy VA, Akhramenko ES. In Vitro Study of the Expression of CD1, CD14, CD25, CD30, CD35, CD95 Receptors by Macrophages of Mice Infected with Mycobacterium tuberculosis. Bull Exp Biol Med. 2021;172(1):42-45doi: 10.1007/s10517-021-05327-9.
Il'in, D. A., Shkurupy, V. A., & Akhramenko, E. S. (2021). In Vitro Study of the Expression of CD1, CD14, CD25, CD30, CD35, CD95 Receptors by Macrophages of Mice Infected with Mycobacterium tuberculosis. Bulletin of experimental biology and medicine, 172(1), 42-45. https://doi.org/10.1007/s10517-021-05327-9
Il'in, D A, et al. "In Vitro Study of the Expression of CD1, CD14, CD25, CD30, CD35, CD95 Receptors by Macrophages of Mice Infected with Mycobacterium tuberculosis." Bulletin of experimental biology and medicine vol. 172,1 (2021): 42-45. doi: https://doi.org/10.1007/s10517-021-05327-9
Il'in DA, Shkurupy VA, Akhramenko ES. In Vitro Study of the Expression of CD1, CD14, CD25, CD30, CD35, CD95 Receptors by Macrophages of Mice Infected with Mycobacterium tuberculosis. Bull Exp Biol Med. 2021 Nov;172(1):42-45. doi: 10.1007/s10517-021-05327-9. Epub 2021 Nov 19. PMID: 34796425.
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Bull Exp Biol Med. 2021 Nov;172(1):42-45. doi: 10.1007/s10517-021-05327-9. Epub 2021 Nov 19.

In Vitro Study of the Expression of CD1, CD14, CD25, CD30, CD35, CD95 Receptors by Macrophages of Mice Infected with Mycobacterium tuberculosis.

Bulletin of experimental biology and medicine

D A Il'in, V A Shkurupy, E S Akhramenko

Affiliations

  1. Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia. [email protected].
  2. Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.
  3. Novosibirsk State Medical University, Ministry of the Health of the Russian Federation, Novosibirsk, Russia.

PMID: 34796425 DOI: 10.1007/s10517-021-05327-9

Abstract

In cultures of peritoneal macrophages (MP) of male BALB/c mice infected with Mycobacterium tuberculosis from the BCG vaccine, the expression of CD1, CD14, CD25, CD30, CD35, and CD95 receptors was studied in vitro 3 months after infection. In MP cultures from intact and infected mice, mononuclear MP predominated (96 and 92%, respectively). Bi- and trinuclear MP in MP cultures from control and infected mice constituted 4 and 8.3% of all MP, respectively. In the cultures of both groups, no obvious correlations between the number of MP expressing CD-receptors and number of nuclei in these cells were found, but the expression of CD14 receptor was more often noted. In cultures from infected animals, hypertrophied MP and enhanced (by several times) expression of all CD-receptors were observed. The increase in the expression of CD-receptor can be determined by activation of plastic processes in hypertrophied MP (in epithelioid and in numerically insignificant polynuclear MP), which is due to the phenomenon of prolonged M. tuberculosis persistence in the vacuolar apparatus of these cells.

© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: CD-receptor expression; macrophages; tuberculosis granulomatosis

References

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